1.云南省农业科学院经济作物研究所;2.云南农业大学植物保护学院
云南省农业基础研究联合专项重点基金(202101BD070001-022);国家自然科学基金(32260505);云南省后备人才项目(202305AC160032);云南种子种业联合实验室项目(202205AR070001);国家马铃薯产业技术体系(CARS-09-P03)
Yunnan Agricultural Joint Foundation (202101BD070001-022);National Natural Science Foundation of China (32260505);Yunnan Talent Project (202305AC160032);Yunnan Seed laboratory Grant (202205AR070001);China Agriculture Research System Grant (CARS-09-P03).
马铃薯晚疫病水平抗性受微效多基因控制,通过表型评价选择效率低下,为创制马铃薯水平抗性种质资源,本研究选用8个亲本材料杂交构建了9个杂交群体,在基于离体叶片抗性鉴定评价晚疫病抗性的基础上,结合SNP位点检测进行分子辅助选择。基于水平抗性基因PCR产物测序,检测了60个抗、感杂交后代材料8个数量抗性基因的19个单核苷酸多态性(SNP)位点,结果表明StAOS2-SNP691、StAOS2-SNP692、StGP28-SNP794、PLOX1-SNP8344有益SNP 位点与抗性存在正向关联,杂交后代群体中抗病材料的有益SNP纯合位点占比为9.56%,高于后代群体中的感病材料(5.65%),同时高于亲本材料(7.89%),联合8个基因19个SNP标记的系统发育分析可将60份材料聚类为3个抗病分支和3个感病分支。结合表型、育种系谱和水平抗性基因分子标记计算与抗晚疫病相关的育种值,筛选出育种值高且具有SNP有益纯合位点的PC2312-3、PC2312-15、PC2313-19等优异单株,为马铃薯水平抗性群体遗传改良提供了材料。
The horizontal resistance to potato late blight is controlled by minorgene with low efficiency through phenotype evaluation. In order to innovate potato germplasm resources with horizontal resistance, this study selected 8 parental materials for cross and constructed 9 hybrid populations. Combined with the late blight resistance evaluation by detached leaf assay, molecular assisted selection was performed on 60 resistant and susceptible hybrid offspring materials by horizontal resistance gene PCR sequencing, 19 single nucleotide polymorphism (SNP) sites of 8 resistance genes were detected. The results showed that StAOS2-SNP691、StAOS2-SNP692、StGP28-SNP794、PLOX1-SNP8344 beneficial SNP loci was positively associated with late blight resistance. the proportion of beneficial SNP homozygous sites in disease resistant materials in the hybrid offspring population was 9.56%, which was higher than that in susceptible materials in the offspring population (5.65%) and higher than that in parental materials (7.89%). A phylogenetic analysis of 19 SNP markers in 8 genes cluster 60 materials into 3 resistant branches and 3 susceptible branches. By combining phenotype, breeding pedigree and molecular markers of horizontal resistance genes, breeding values related to resistance to late blight were calculated, and excellent individual plants such as PC2312-3, PC2312-15, and PC2313-19 with high breeding values and beneficial SNP homozygous loci were screened, providing materials for genetic improvement of potato horizontal resistance populations.