The quantitative resistance to potato late blight is controlled by multiple genes each with limited effectiveness as determined through phenotype evaluation. In order to develop novel potato germplasm with enhanced quantitative resistance， this study deployed eight parental lines to construct nine hybrid populations. Using detached leaf assays for late blight resistance evaluation coupled with molecular marker-assisted selection， we analyzed 60 resistant and susceptible hybrid offspring through PCR sequencing of quantitative resistance genes， identifying 19 single nucleotide polymorphism （SNP） sites across eight resistance genes. These results demonstrated that specific favorable allele （StAOS2-SNP691， StAOS2-SNP692， StGP28-SNP794， PLOX1-SNP8344） showed positive correlations with late blight resistance. The proportion of favorable homozygous allele in resistance offspring （9.56%） significantly exceeded that in susceptible offspring （5.65%） and parental materials （7.89%）. Phylogenetic analysis based 19 SNP markers from eight genes clustered the 60 materials into three resistant and three susceptible branches. By integrating phenotypic data， breeding pedigree and resistance genes markers， we calculated breeding values for late blight resistance and identified superior genotypes （PC2312-3， PC2312-15， and PC2313-19） exhibiting both high breeding values and favorable SNP homozygous allele， therefore providing valuable genetic materials for improving potato resistance.