The genome sequencing of Common bean has been completed, but the research on its important genetic traits and genetic transformation is being carried out gradually. In this study, the WOX gene nucleotide sequence, gene structure, conserved domain, chromosome location and cis-acting elements of common bean. were identified in the whole genome. Genomic collinearity and phylogenetic tree analysis of WOX gene family members in Common bean, Mung bean, Soybean and Arabidopsis were performed. The callus induction system of two common bean. varieties was successfully established. Differentially expressed WOX genes related to callus induction rate were identified by comparison of transcriptome and qRT-PCR analysis. The results showed that the stem callus of two Common bean varieties Long17-3965 and Long10 were successfully induced by MS+0.4mg/L 6-BA+1mg/L 2,4-D and MS+0.6mg/L 6-BA+1mg/L 2,4-D, and the highest healing rate was obtained. The whole genome identified 18 WOX gene family members in common bean, distributed on 10 chromosomes. Phylogenetic tree analysis showed that 18 Common bean, 15 Arabidopsis, 58 Mung bean and 33 Soybean WOX gene family members could be divided into three major groups. The conserved domains of WOX genes in three major groups mainly include HD (homeodomain) domains. PvWOXs gene promoters mainly contained 28 different types of cis-acting elements such as "meristem formation correlation" and"auxin response correlation". Genomic collinearity analysis among 4 species was found that most of gene containing the number of synonymous mutation greater than nonsynonymous mutaion in nucleotide sequence. Using MS + 0.4 mg/L 6-BA + 1 mg/L 2,4-D and MS + 0.6 mg/L 6-BA + 1 mg/L 2,4-D medium, a successful system for inducing callus tissue from the hypocotyls of Long 17-3965 and Long 10 was established. Transcriptome analysis revealed 8 differentially expressed WOX genes; correlation analysis showed that PvWOX1/15/16 was related to callus induction rate, with PvWOX1/15 downregulated during callus formation and PvWOX16 upregulated. The 3 WOX differentially expressed gene were identified by comparative transcriptome analysis. qRT-PCR identification results consistented with PvWOX1/15/16 gene expression value FPKM in transcriptomic data.Meantime, PvWOX1/15/16 gene expression level were signifcantly correlated with the rate of callus induction. According to the bove results, it suggested that PvWOX1/15/16 gene can be the potential target genes for the research on genetic transformation in Common bean. Further, this study provides key gene resources for establishing efficient regeneration system and genetic transformation of Common bean in the future.