Rice of direct-seeding is characterized by labor-saving, labor-saving and low-cost production, and the planting area is expanding year by year.Water shortage is one of the most important problems in rice production.Accurate identification of direct seeding tolerance of rice germplasm resources is of great significance to direct seeding tolerance breeding in China. Direct-seeding resistance in the field is a complex quantitative trait. Accurate phenotypic identification is critical to discover the genes and explore genetic basis for quantitative trait. However, the accurate identification of direct-seeding resistance for rice germplasm in the field has consistently been a bottleneck for researcher and a core technical for direct-seeding resistance breeders. On the basis of systematic direct-seeding resistance research in the previous work, we also have spent many years exploring and optimizing the conditions for assessing direct-seeding resistance in the field. The standard technical regulations for evaluating direct-seeding resistance in the field were summarized and normalized. It included general requirements, land preparation before sowing, seed preparation, sowing, fertilization,water management, prevention of diseases, pests and weeds, and evaluation of direct-seeding resistance in the field at seedling stage. The regulations provided technical reference for identification of direct-seeding resistance in the field at seedling stage in rice or other crops. It was suitable for the resistance evaluation of rice to direct-seeding during the spring in northwest region (from mid-March tolate June).