Plastid glyceraldehyde-3-phosphate dehydrogenase （GAPCp） plays an important role in plant growth and development， energy metabolism， and stress response.In this study，we cloned the open reading frame （ORF） sequence of the IbGAPCp1 gene from sweetpotato variety 'Lizixiang'. Subcellular localization studies showed that the IbGAPCp1 protein is targeted to the chloroplasts. Quantitative real-time PCR analysis showed that IbGAPCp1 is ubiquitously expressed across sweetpotato tissues， with the highest transcripts detected in leaves. The expression of IbGAPCp1 gene increased gradually with the development of sweetpotato tuberous root and reaching its peak at 105 days. In order to further explore the regulatory mechanism of IbGAPCp1 gene， we cloned a 1940 bp sequence of the IbGAPCp1 gene promoter， designated as Pro-IbGAPCp1. The prediction of cis-acting elements showed that Pro-IbGAPCp1 contains several elements related to photoresponse， hormone response and meristem expression. Expression profiling under drought and salt stress showed that IbGAPCp1 is responsive to both drought and salt stresses. Furthermore， yeast one-hybrid screening identified 35 upstream transcriptional regulators interacting with the promoter region of IbGAPCp1， including ethylene-insensitive protein 2， zinc finger CCCH domain-containing protein 47 and MYB44， factors that are known to modulate plant growth and development， secondary metabolism， energy production， and stress responses. This study provides a theoretical basis for future elucidating the functional role of IbGAPCp1 in sweetpotato abiotic stress tolerance.