1.中国农业科学院作物科学研究所;2.青海大学农林科学院;3.云南省农业科学院粮食作物研究所;4.贵州省农业科学院园艺研究所;5.四川省农业科学院作物研究所;6.青海大学高原生态与农业国家重点实验室
国家重点研发计划项目(2021YFD1200105-02);国家自然科学基金(32272134);国家现代农业产业技术体系-食用豆(CARS-08); 作物基因资源与育种全国重点实验室;中国农业科学院科技创新工程(01-ICS-07)。
1.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences;2.Qinghai Academy of Agricultural and Forestry Sciences,Qinghai University;3.Institute of Grain Crops,Yunnan Academy of Agricultural Sciences;4.Institute of Horticulture,Guizhou Academy of Agricultural Sciences;5.Crop Research Institute,Sichuan Academy of Agricultural Sciences;6.State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University
The National Key Technologies R&D Program of China(2021YFD1200105-02); National Natural Science Foundation of China (32272134); China Agriculture Research System of MOF and MARA-Food Legumes (CARS-08); State Key Laboratory of Crop Gene Resources and Breeding; Science and Technology Innovation Project of CAAS (01-ICS-07).
救荒野豌豆(Vicia sativa L.)是一种一年生高蛋白冷季豆类,具有耐寒、耐旱、耐瘠薄的特性,被广泛用作绿肥和饲料,在作物轮作系统中发挥了重要作用。过去,我国对救荒野豌豆种质资源缺乏系统的调查收集和鉴定评价,为了进一步探索其利用价值,选育高产优异种质,本研究在贵州、青海、川渝和云南地区采集了214份救荒野豌豆种质资源,进行田间试验,调查表型数据进行多样性分析和鉴定评价。结果表明,救荒野豌豆种质资源的16个农艺性状遗传多样性丰富,其中单株粒数变异系数最高为70%;根据相关性分析,大部分性状之间呈极显著正相关;对来自不同地区的救荒野豌豆进行性状差异统计分析得出,贵州与川渝地区的种质性状差异不显著,青海、云南地区的种质与其他地区的种质在大部分性状上差异显著;主成分分析将16个农艺性状指标集中在累计贡献率达到81.1%的5个主成分,其中第一主成分主要与单株粒重、单株粒数、干荚长和单株荚数等产量性状相关,而第二主成分主要与百粒重、粒长和粒宽等籽粒大小相关;通过PCA三维立体图聚类分析发现,不同地区的种质在表型上存在明显分化,云南和青海的种质单独聚类成簇,而贵州和四川的种质聚在一起,说明川黔两地的资源在形态性状上较为相似。通过进一步的表型鉴定评价,筛选出两份救荒野豌豆多荚多粒高产种质,单株荚数大于100个,单株粒数大于800粒,单株粒重大于10克,为救荒野豌豆种质资源的保护利用和育种改良提供了宝贵资源和理论支撑。
Common vetch (Vicia sativa L.) is an annual legume with high protein content, well-adapted to cold climates, drought conditions, and poor soil fertility. It is extensively utilized as a green manure and livestock feed, playing a crucial role in crop rotation systems. However, systematic investigation and collection as well as identification and evaluation of germplasm resources in common vetch have been lacking in China until now. In order to further explore its utilization potential and select high-yielding and superior germplasm varieties, this study collected 214 germplasm resources of common vetch from the regions of Guizhou, Qinghai, Sichuan-Chongqing, and Yunnan. Field trials were conducted and phenotypic data was investigated for diversity analysis and identification. The results revealed a rich genetic diversity in the 16 agronomic traits of the germplasm resources, with seed number per plant (SNPP) exhibiting the highest coefficient of variation at 70%. Correlation analysis indicated predominantly positive associations among most of the characters. Statistical analysis was performed to comparing trait differences between common vetch from different regions, which demonstrated no significant distinction between Guizhou and Sichuan-Chongqing areas, while notable variations were observed between Qinghai and Yunnan germplasm and other regions across multiple traits. Principal component analysis (PCA) condensed the 16 agronomic traits into five principal components, which accounted for a cumulative contribution rate of 81.1%. The first principal component primarily encompassed yield-related traits such as grain weight per plant, grain number per plant, dry pod length, and pod number per plant; whereas the second principal component mainly represented grain size attributes including hundred-grain weight, grain length, and grain width. Three-dimensional cluster analysis based on PCA exhibited distinct phenotypic differentiation among germplasm from different regions. Germplasm from Yunnan and Qinghai formed separate clusters while those from Guizhou and Sichuan grouped together, indicating morphological trait similarities within Sichuan and Guizhou resources. Through further phenotypic identification and evaluation, two high-yielding germplasm with a pods per plant (PPP) exceeding 100, seed number per plant (SNPP) surpassing 800, and seed weight per plant (SWPP) exceeding 10 grams were selected, which provides valuable resources and theoretical support for the conservation, utilization, and breeding enhancement of common vetch germplasm resources.