1.南阳师范学院水资源与现代农业学院,河南南阳 473061;2.河南省菌类食品工程技术研究中心,南阳 473061
研究方向为作物抗逆种质资源发掘及抗逆分子机理,E-mail: 643147683@qq.com
陈吉宝,研究方向为作物抗逆种质资源发掘及抗逆分子机理,E-mail: chenjibao2012@163.com
河南省科技攻关计划项目(212102110248)
1.College of Water Resources and Modern Agriculture,Nanyang Normal University,Nanyang 473061,Henan;2.Engineering Technology Research Center of Fungal Food in Henan Province,Nanyang 473061
Science and Technology Project of Henan Province(212102110248)
普通菜豆(Phaseoluus vulgaris L.)是主要食用豆类作物之一,干旱是限制普通菜豆生长最严重的非生物胁迫因子,培育耐旱种质是减少干旱对普通菜豆生长不利影响的重要措施。本研究利用自然干旱法,在苗期对60份普通菜豆种质进行干旱处理,并测定叶片相对含水量、水分利用效率和叶绿素荧光参数等12项生理指标,分别以幼苗萎蔫指数法和耐旱隶属函数综合值法(D值法)对60份普通菜豆种质进行耐旱性评价,筛选耐旱种质。结果表明,依据D值法,F00000117、F00002179、F00003370、F00004321、F00000518、F00004381和南阳黑共7份种质被判定为高耐旱种质,其中除南阳黑和F00004381外的5份种质也通过幼苗萎蔫指数法被判定为高耐旱种质;F00001280、F00003346、F00004357、F00004392、F00005237、F00005243共6份种质用幼苗萎蔫指数法被判定为高敏感种质,其中除F00004357外的5个品种用D值法也被判定为高敏感种质。以上结果说明两种方法对于高耐旱种质和高敏感种质的判定有较好的一致性。本研究获得的高耐旱种质和高敏感种质可应用于普通菜豆耐旱机理研究和耐旱品种选育。
The common bean (Phaseoluus vulgaris L.) is one of the main edible legumminous crops,drought is one of the most critical abiotic stress constraints limiting common bean growth and productivity. Cultivating drought-tolerant germplasm is an important measure to reduce the adverse effects of drought on the growth of common bean. In this study, 60 representative common bean varieties were evaluated through natural drought stress experiments. Comparative physiological analysis between stressed and control groups were performed at 12 leaf physiology characters, such as leaf electrical conductivity, water use efficiency and Photosysm II chlorophyll fluorescence parameters. Drought tolerance was assessed by two complementary approaches, visual seedling wilting grade and quantitative drought tolerance subordinate function value (D value). The results identified seven highly drought-tolerant varieties (F00000117, F00002179, F00003370, F00004321, F00000518, F000004381, and Nanyang Black) based on D value. Notably, five of these varieties (excluding Nanyang Black and F00004381) were consistently ranked as most tolerant by applying wilting index. Conversely, six highly susceptible varieties (F00001280, F00003346, F00004357, F00004392, F00005243, and F00005237) showed severe wilting, with five (F00001280, F00003346, F00004392, F00005243, and F00005237) also exhibiting the lowest D values. The strong concordance between seedling wilting and D assessments validates both methods for drought tolerance screening. The extreme varieties (drought-tolerant vs. drought-susceptible) identified in this study provide valuable germplasm resources for drought tolerance mechanism analysis and breeding.
蔡德宝,杨树琼,马郡妍,等.普通菜豆苗期耐旱性鉴定[J].植物遗传资源学报,2025,26(7):1355-1369.
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