南阳师范学院
河南省科技攻关计划项目(212102110248)
Nanang Normal University
Science and Technology Project of Henan Province(212102110248)
普通菜豆(Phaseoluus vulgaris L.)是主要食用豆类作物之一,干旱是限制普通菜豆生长的最严重非生物胁迫因子,培育耐旱品种是减少干旱对普通菜豆生长不利影响的重要措施。本研究利用自然干旱法,在苗期对60个普通菜豆品种进行干旱处理,并测定叶片相对含水量、水分利用效率和叶绿素荧光参数等12项生理指标,分别以幼苗萎蔫指数法和耐旱隶属函数综合值法(D值法)对60个普通菜豆品种进行耐旱性评价,筛选耐旱品种。结果表明,依据D值大小,F00000017、F00000518、F00002179、F00003370、F00004321和“南阳黑”共6个品种被判定为高耐旱品种,其中除“南阳黑”外的5个品种也被幼苗萎蔫指数法判定为高耐旱品种;F00001280、F00003346、F00005237、F00004357、F00004392、F00005243共6个品种用幼苗萎蔫指数法被判定为高水敏性品种,其中前3个品种用D值法也被判定为高水敏性品种,后3个为水敏性品种。以上结果说明两种方法对于高耐旱品种和高水敏性品种的判定有很好的一致性。本研究获得高耐旱品种和高敏性品种可应用于普通菜豆耐寒机理研究和耐旱品种选育。
Drought is the most important abiotic stress factor limiting the growth of common bean. In this study, 60 representative common bean germplasms were treated with drought stress using natural drought method. 12 leaf physiology characters, such as leaf electrical conductivity, water use efficiency (WUE) and 5 the Photosysm II chlorophyll fluorescence parameters, were analyzed between drought stress treatment group and control group. The drought tolerance of 60 common beans was evaluated by seedling wilting grade and drought tolerance subordinate function value (D value). The results showed that 6 varieties,including F00000017, F00000518, F00002179, F00003370. F00004321 and Nanyang Black variety, were classified as the strongest drought tolerance variety based on the leaf wilting index. Among the 6 varieties, 5 varieties except for Nanyang Black variety were also identified as the strongest drought tolerance variety based on the D value. 6 varieties, including F00001280、F00003346、F00005237、F00004357、F00004392 and F00005243, were classified as the strongest drought-susceptible variety based on the leaf wilting index. F00001280、F00003346、F00005237 were also identified as the strongest drought-susceptible variety based on D value. These results indicated that there were higher consistency in identifying the common bean drought tolerance between the seedling wilting grade method and drought tolerance subordinate function value (D value) method. The strongest drought tolerance varieties and the strongest drought-susceptible varieties identified in this study could be used in the study of common bean drought tolerance mechanism and breeding.