1.四川省农业科学院作物研究所(四川省种质资源中心)/粮油作物绿色种质创新与遗传改良四川省重点实验室;2.四川省农业科学院作物研究所四川省种质资源中心/粮油作物绿色种质创新与遗传改良四川省重点实验室;3.四川省种子站
四川省蔬菜育种攻关项目“蚕豆蔬菜育种新材料创制与突破性新品种选育”(2021YFYZ0022);国家现代农业产业技术体系项目“四川豆类杂粮创新团队”(SCCXTD-2022-20); 第三次全国农作物种质资源普查与收集行动(111821301354052034); 四川省农业科学院“1+9揭榜挂帅”项目(1+9KJGG001); 四川省财政专项(1+3 ZYGG001)
1.Crop Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Provincial Germplasm Resources Center);2.Crop Research Institute,Sichuan Academy of Agricultural Sciences Sichuan Provincial Germplasm Resources Center/Environment-friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province;3.Sichuan Seed Station
The key project of vegetable breeding of Sichuan "Vegetable faba bean breeding new material creation and breakthrough new variety breeding " (2021YFYZ0022); National Modern Agricultural Industry Technology System Project "Sichuan innovation team of bean and miscellaneous grains" (SCCXTD-2022-20); The Third National Campaign of Crop Germplasm Census and Collection (111821301354052034); Sichuan Academy of Agricultural Sciences Project "1+9 Unveiling the List" (1+9KJGG001); Sichuan Provincial Finance Department (1+3 ZYGG001)
四川是蚕豆资源遗传多样性较为丰富的省份之一,本文以82份四川蚕豆地方种质资源为研究对象,通过遗传多样性指数、变异系数、相关性分析、聚类分析和主成分分析,鉴定评价了四川蚕豆地方资源的多样性水平,筛选了优异种质资源。结果表明,82份蚕豆种质资源的株高、百粒重、叶形、鲜茎色、赤斑病抗性与褐斑病抗性遗传多样性丰富;除荚宽和生育日数外的11个农艺性状变异系数在11.70%~38.39%之间,每荚粒数、结荚节数、单株荚数和单株粒数的变异系数较大。相关分析结果显示,株高、百粒重、有效分枝、结荚节数、单株荚数和单株粒数均与单株粒重呈显著或极显著正相关关系。聚类分析将82份蚕豆种质资源聚为四类,I、IV类产量正相关性状表现较好。主成分分析提取了累计百分率达79.858%的5个主成分因子,有效分枝、结荚节数、单株荚数对PCA1贡献较大为优异资源评价的关键农艺性状,筛选了主成分因子加权得分>1的7份种质资源作为优异资源。本研究结果反映了四川蚕豆地方种质资源的综合表现,为优异资源保护、挖掘和利用提供了数据支撑。
Sichuan was one of the provinces with rich genetic diversity of faba bean resources. In this paper, 82 Sichuan faba bean local germplasm resources were used as the research object. The diversity level of Sichuan faba bean local resources was identified and evaluated by genetic diversity index, variation coefficient, correlation analysis, cluster analysis and principal component analysis, and excellent germplasm resources were screened. The results showed that the genetic diversity of plant height, 100-seed weight, shape of the leaflets, color of the fresh stem, resistance of chocolate spot and resistance to ascochyta blight of faba bean germplasm resources was rich. The variation coefficient 11 agronomic traits except pod width and growth days ranged from 11.70 % to 38.39 %, and the variation coefficient of seed per pod, number of pod nodes, pods per plant and seeds per plant was larger. The correlation analysis results showed that plant height, 100-seed weight, number of effective branches, number of pod nodes, pods per plant and seeds per plant were significantly or extremely significantly positively correlated with seed weight per plant. Cluster analysis clustered 82 faba bean germplasm resources into four categories, and class I and IV yield-related traits performed better. Five principal component factors with a cumulative percentage of 79.858 % were extracted by principal component analysis. The effective branch number, number of pod nodes and pods per plant contributed greatly to PCA1, which were the key agronomic traits for excellent resource evaluation. Seven germplasm resources with a weighted score of principal component factor > 1 were selected as excellent germplasm. The results of this study reflected the comprehensive performance of faba bean local germplasm resources in Sichuan, and provided data support for the protection, excavation and utilization of excellent resources.