黑龙江八一农垦大学
国家自然科学基金(32341033);中国博士后科学基金(2024MD753900);黑龙江省重点研发计划(GZ20230024,GZ20220070);黑龙江省“双一流”学科协同创新成果项目(LJGXCG2024-F20);黑龙江八一农垦大学研究生创新科研项目(YJSCX2023-Y08);黑龙江八一农垦大学大学生创新创业训练计划项目(202310223159)
National Natural Science Foundation of China (32341033); China Postdoctoral Science Foundation(2024MD753900); Key Research and Development Program of Heilongjiang Province(GZ20230024, GZ20220070); Heilongjiang Province "Double First Class" Discipline Collaborative Innovation Achievement Project, LJGXCG2024-F20; Graduate Innovative Research Project of Heilongjiang Bayi Agricultural Reclamation University(YJSCX2023-Y08);Heilongjiang Bayi Agricultural Reclamation University College Student Innovation and Entrepreneurship Training Program Project(202310223159)
植物谷氨酸受体通道蛋白(Glutamate Receptor-like channels,GLRs)是一类参与细胞代谢、种子萌发、植物生长发育及长距离信息传递的Ca2+通道蛋白。本研究通过对大豆蛋白组进行全蛋白组鉴定,共获得29个大豆GLR成员。系统进化关系表明大豆GLR基因分为GroupⅠ-Ⅲ,同一亚家族成员基因结构、保守结构域和保守基序分布相对保守。顺式作用元件分析证实大豆GLRs启动子区包含与激素、胁迫、光照以及植物生长发育相关调控元件。组织和胁迫表达模式分析表明,GLR基因在大豆各组织均有较高表达,且显著受盐碱胁迫诱导表达。qRT-PCR分析显示GmGLR3.13/15/4.8/4.9/4.10基因盐和碱胁迫下呈现显著差异,表明其可能在胁迫应答方面具有重要作用。本研究为深入探究大豆GLR基因在逆境应答中的作用提供重要的理论依据。
Glutamate receptor like channels (GLRs) are a type of Ca2+channel proteins involved in cellular metabolism, seed germination, plant growth and development, as well as long-distance signal transmission. Here, in this study, a total of 29 soybean GLR members were identified through a comprehensive proteomic identification of soybean. Their phylogenetic relationship suggests that these soybean GLR genes can be classied into three subfamilies, members within the same subfamily exhibits conserved gene structure and motif compositions. Cis-acting elements analysis indicates that the promoter regions of soybean GLRs contain a variety of regulatory elements related to hormones, stress, light, and plant growth and development. The analysis of tissue and stress expression patterns shows that GLR genes are highly expressed across various tissues in soybeans and involved in salt and alkaline stress. GmGLRs exhibit significant variations under salt and alkaline stress by qRT-PCR analysis, suggesting that GmGLR3.13/15/4.8/4.9/4.10 may play an important role in stress response.In summary, the results of this study provide an important theoretical basis for further investigating the role of soybean GLR genesin stress responses.