1.郑州大学农学院;2.河南省作物分子育种研究院
河南省联合基金项目(222301420026),
Key projects of Henan Province(No. 222301420026)
叶片是植物进行光合作用、呼吸作用和蒸腾作用的关键器官,其形态建成受遗传、环境和进化因素的共同影响,同时其大小也会影响作物的生长发育、产量和品质。研究叶片的发育机制、了解环境因素对叶片发育的影响以及明确适宜叶片大小范围,对农业生产具有重要意义。然而,在植物叶片发育研究过程中仍存在许多问题,例如基因互作网络、多环境因子耦合效应、适宜叶片大小范围的划分及育种应用等关键问题仍需突破。因此,本文旨在总结近年来对各种植物叶片发育调控机制、影响因素的研究概况,分析关于植物叶片大小的当前研究状态、趋势和关键问题,以期为后续其他植物叶片研究提供参考,具体包括以下几点:一是叶片形态建成机制,解析了叶片形态建成的三个阶段;二是叶片发育的调控机制,主要总结了在细胞水平和分子水平上的调控机制;三是影响叶片形态的因素,包括遗传因素和环境因素;四是关于叶片研究的相关技术,包括一些前沿科技、传统实验手段、实验仪器及分析软件,总结了这些技术在叶片发育研究中的应用。
Leaves are pivotal organs for photosynthesis, respiration, and transpiration in plants. Their morphological development is jointly influenced by genetic, environmental, and evolutionary factors, while their size significantly impacts crop growth, yield, and quality. Investigating leaf developmental mechanisms, understanding environmental influences on leaf formation, and defining the optimal leaf size range are of great importance for agricultural production. However, numerous challenges remain in plant leaf development research, including gene interaction networks, coupled effects of multiple environmental factors, classification of optimal leaf size ranges, and applications in breeding-all of which require further breakthroughs. Therefore, this review employs a literature-based research approach to summarize recent advances in the regulatory mechanisms and influencing factors of leaf development across various plant species. It analyzes the current research status, trends, and key issues concerning leaf size, aiming to provide a reference for future studies. The main aspects covered include:(1)Mechanisms of leaf morphogenesis, elucidating the three stages of leaf shape formation. (2)Regulatory mechanisms of leaf development, primarily summarizing cellular and molecular-level control pathways. (3)Factors influencing leaf morphology, including genetic and environmental determinants. (4)Technologies applied in leaf research, encompassing cutting-edge techniques, traditional experimental methods, instruments, and analytical software, along with their applications in leaf development studies.
