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首页 > 过刊浏览>2025年第26卷第11期 >2164-2180. DOI:10.13430/j.cnki.jpgr.20250618001 优先出版
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30份木薯种质资源表型性状遗传多样性分析及综合评价
DOI:
10.13430/j.cnki.jpgr.20250618001
CSTR:
作者:
作者单位:

1.云南省农业科学院热带亚热带经济作物研究所,保山 678000;2.云南省农业科学院国际农业研究所,昆明 660205

作者简介:

研究方向为木薯资源与育种,E-mail: liyuexiann2008@126.com

通讯作者:

刘光华,研究方向为木薯资源与育种,E-mail: rjslgh@vip.126.com

中图分类号:

基金项目:

云南省重点研发计划(202503AK140055);现代农业产业技术体系(CARS-11-YNLGH);云南省院士专家工作站(202305AF150004);热带作物生物育种全国重点实验室科研项目(SKLTCB-YAAS-2025-02)


Genetic Diversity Analysis and Comprehensive Evaluation of Phenotypic Traits in 30 Cassava Germplasm Resources
Author:
Affiliation:

1.Tropical and Subtropical Economic Crops Research Institute, Yunnan Academy of Agricultural Sciences, Baoshan 678000;2.International Agricultural Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 660205

Fund Project:

Key R & D Program of Yunnan Province,China (202503AK140055); Modern Agricultural Industry Technology System(CARS-11-YNLGH); Yunnan Province Academician Expert Workstation(202305AF150004); Tropical Crops Biological Breeding National Key Laboratory Research Project(SKLTCB-YAAS-2025-02)

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    摘要:

    以30份木薯种质资源为研究对象,测定40个表型性状,综合运用多样性指数、相关性分析和主成分分析等方法对木薯种质表型性状进行遗传多样性分析及系统评估,并筛选优异的木薯种质资源。结果表明,24个质量性状遗传多样性指数在0.393~2.056,广义遗传力在91.16%~100%,其中遗传多样性指数最高的是嫩茎颜色,最低的是烂根情况,且遗传多样性指数大于1的性状有第一片完全展开叶的颜色、顶端未展开嫩叶颜色、块根形状等12个性状;16个数量性状的变异系数在12.17%~61.66%,遗传多样性指数在0.7614~2.0883,广义遗传力在84.19%~99.58%,其中遗传多样性指数最高的是收获指数,最低的是叶片裂叶数,除叶片裂叶数外,株高、鲜薯淀粉含量、叶面积等15个性状的遗传多样性指数均大于1,说明30份木薯类型多样、遗传多样性丰富。株高与单株块根鲜重、成熟主茎内皮颜色、块根肉质颜色等呈一定的相关性。主成分分析提取到13个主成分,累计贡献率达到了82.23%,其中株高、茎粗、顶端未展开嫩叶颜色、单株块根鲜重等21个性状贡献较大。逐步回归分析法确定了顶端未展开嫩叶颜色、块根分布等7个性状对木薯综合性状影响显著。利用隶属函数综合评价法筛选出H588、GR6、GR3等排名前10的优异种质资源。聚类分析将30份种质分为4大类,第Ⅰ类为高产、高淀粉类群;第Ⅱ类为株高中等、结薯集中、收获指数较高、开花多的杂交育种材料类群;第Ⅲ类为株型直立、高产类群;第Ⅳ类为分枝低、植株偏小、淀粉含量适中的低位分枝类群。研究结果可为木薯种质资源高效发掘利用和新品种选育提供参考。

    Abstract:

    A study was conducted on thirty cassava germplasm accessions through evaluating 40 phenotypic traits. Genetic diversity analysis and systematic phenotypic evaluation were assessed by applying diversity indices, correlation analysis, and principal component analysis, leading to the identification of excellent cassava germplasm resources. Results showed that the genetic diversity index (H′) of 24 qualitative traits ranged from 0.393 to 2.056, with broad-sense heritability (H2) varying between 91.16% and 100.00%. The highest H′ value was observed for the color of young stems, while the lowest was for the situation of rotten roots. Twelve traits exhibited H′ values greater than 1, including color of the first fully expanded leaf, color of the top unexpanded tender leaves, and shape of the tuberous roots. For the 16 quantitative traits, the coefficient of variation (CV) ranged from 12.17% to 61.66%, the genetic diversity index from 0.7614 to 2.0883, and the broad-sense heritability from 84.19% to 99.58%. The highest H′ value was observed for the harvest index, and the lowest was for the number of lobes and leaflets. With the exception of the number of lobes, 15 traits (including plant height, fresh root starch content, and leaf area) all have genetic diversity indices greater than 1. This indicates that the 30 cassava types are diverse and have rich genetic diversity. Plant height was significantly correlated with fresh tuber weight per plant, the color of the inner and outer bark of the main stem, and the color of the tuberous root flesh. Principal component analysis extracted 13 principal components, with a cumulative contribution rate of 82.23%. Among them, plant height, stem diameter, the color of the top unexpanded leaves, and fresh tuber weight per plant had relatively large contribution rates. Stepwise regression analysis identified seven independent traits, including the color of the top unexpanded tender leaves and the distribution of tuberous roots, which significantly affected the comprehensive traits of cassava. The excellent germplasm resources ranked in the top 10 were selected by the comprehensive evaluation method of membership function, including H588, GR6, and GR3. Cluster analysis divided the 30 germplasm resources into four major categories: Group Ⅰ comprised high-yield and high-starch genotypes; Group Ⅱ included hybrid breeding materials with medium plant height, concentrated tuberization, high harvest index, and abundant flowering; Group Ⅲ consisted of erect plant types with high yield potential; and Group Ⅳ encompassed low-branching genotypes with compact plant architecture and moderate starch content. These results can provide valuable insights for efficient exploration and utilization of cassava germplasm resources and support future breeding of new varieties.

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李月仙,段春芳,宋记明,等.30份木薯种质资源表型性状遗传多样性分析及综合评价[J].植物遗传资源学报,2025,26(11):2164-2180.

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  • 收稿日期:2025-06-18
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  • 在线发布日期: 2025-10-21
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