1.河北农业大学农学院,保定071000;2.黑龙江省农业科学院克山分院,齐齐哈尔161600
研究方向为作物遗传育种,E-mail:gzc11486@163.com;
宋继玲,研究方向为马铃薯种质资源研究与利用,E-mail:jl_song929@126.com
崔江慧,研究方向为作物遗传育种,E-mail:cjianghui521@126.com
农业农村部物种保护项目(19240423);科技部、财政部国家科技资源共享服务平台项目(NCGRC-2024-44)
1.College of Agronomy, Hebei Agricultural University, Baoding 071000;2.Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161600
Species Conservation Project of the Ministry of Agriculture and Rural Affairs(19240423); National Science and Technology Resource Sharing Service Platform Project of the Ministry of Science and Technology and the Ministry of Finance (NCGRC-2024-44)
为了分析马铃薯种质资源的多样性,筛选优异种质,以197份马铃薯种质资源为研究对象,综合运用Simpson指数、 Shannon-Wiener指数、变异系数、相关性分析、主成分分析、聚类分析、分段线性回归、多元线性回归分析等对17个表型性状进行鉴定与评价。结果表明,12个质量性状的Simpson指数介于0.441~0.714,Shannon-Wiener指数范围为0.752~1.410,薯肉色多样性最高,存在丰富的变异。5个数量性状中还原糖含量的变异系数达到31.61%,而株高相对较小,为14.21%;Simpson指数介于0.845~0.859,Shannon-Wiener指数在2.008~2.077之间,淀粉含量、茎粗等性状的Shannon-Wiener指数较高,具有较大的遗传改良潜力;茎粗在两年间稳定性较强,还原糖含量为环境敏感型性状。相关性分析表明株高与茎粗、块茎产量、淀粉含量之间呈正相关但未达到显著,株高与还原糖、块茎产量与淀粉含量之间分别呈显著、极显著负相关。对数量性状采用主成分分析提取了3个主成分因子,累积贡献率为66.408%。利用隶属函数与主成分分析结合计算综合得分,筛选出克新27号、华薯13号、川芋117等10个优异种质资源。通过对 197 份参试种质综合得分C 值进行聚类分析,将其划分为4个类群,各聚类类群间表现差异显著。结合分段线性回归分析和多元线性回归分析,将复杂的非线性关系转化为多个简单线性区间的组合,株高、茎粗和块茎产量可作为评价马铃薯综合值的关键指标。本研究结果为马铃薯种质的利用及品种选育提供科学依据。
To analyze the diversity of potato germplasm resources and screen elite germplasm, 197 potato germplasm resources were used as research objects. Seventeen phenotypic traits were identified and evaluated by comprehensively applying Simpson index, Shannon-Wiener index, coefficient of variation, correlation analysis, principal component analysis, cluster analysis, piecewise linear regression, and multiple linear regression analysis. The results showed that the Simpson index of 12 qualitative traits ranged from 0.441 to 0.714, and the Shannon-Wiener index ranged from 0.752 to 1.410. Tuber flesh color exhibited the highest diversity with abundant variations. Among the 5 quantitative traits, the coefficient of variation of tuber reducing sugar content reached 31.61%, while plant height was relatively small (14.21%). The Simpson index of 5 quantitative traits ranged from 0.845 to 0.859, and the Shannon-Wiener index was ranged from 2.008 to 2.077. Traits such as starch content and stem diameter had higher Shannon-Wiener index, indicating greater potential for genetic improvement. Stem diameter showed strong stability over two years, while tuber reducing sugar content was an environment-sensitive trait. Correlation analysis revealed that plant height was positively correlated with stem diameter, yield, and starch content, but the correlations were not significant. Plant height was significantly negatively correlated with tuber reducing sugar content, and tuber yield was extremely significantly negatively correlated with tuber starch content. For quantitative traits, principal component analysis extracted 3 principal component factors with a cumulative contribution rate of 66.408%. By combining the membership function with principal component analysis to calculate comprehensive scores, 10 elite germplasm resources were screened out, including Kexin No.27, Huashu No.13, and Chuanyu No.117. Cluster analysis based on the comprehensive score (C value) of the 197 tested germplasm divided them into 4 clusters, with significant differences among clusters. Through piecewise linear regression and multiple linear regression analysis, complex nonlinear relationships were converted into combinations of multiple simple linear intervals. Plant height, stem diameter, and tuber yield could be used as key indicators for evaluating the comprehensive value of potatoes. The results of this study provide a scientific basis for the utilization of potato germplasm and variety breeding.
高志昌,杨梦平,张金鹏,等.马铃薯种质资源表型性状鉴定与综合评价[J].植物遗传资源学报,2025,26(11):2131-2144.
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