Gummy stem blight is one of the major diseases threatening watermelon production. Identifying and utilizing resistance genes to gummy stem blight is of great significance for the innovation of disease resistant watermelon germplasm and breeding of resistant watermelon varieties. In this study， a recombinant inbred line （RIL） population derived from the cross between the gummy stem blight resistant germplasm PI189225 and the susceptible germplasm K3 was used as the experimental materials. Bulked segregant analysis （BSA） combined with whole genome resequencing of the parents and resistant and susceptible bulks was performed to identify candidate genomic regions associated with gummy stem blight resistance. Additionally， transcriptome sequencing （RNA-seq） data were integrated to mine candidate genes. The results showed that BSA-seq analysis identified genome regions significantly associated with gummy stem blight resistance on chromosome 5 and 10， with a total length of 8.18 Mb containing 681 genes. Functional analysis revealed that these genes are primarily involved in biological processes such as plant-pathogen interaction and phenylpropanoid biosynthesis. Further analysis using InDel markers and recombinant individuals narrowed down the resistance interval to a 2.45 Mb region on chromosome 10， flanked by the markers Chr10_30103333 and Chr10_32554279. Combined with differentially expressed genes in watermelon responding to gummy stem blight infection， six candidate genes were identified on chromosome 10. qRT-PCR analysis indicated that the expression of all six candidate genes was induced by gummy stem blight pathogen. Among them， Cla97C10G200140 and Cla97C10G202140 were highly expressed in susceptible parent， whereas Cla97C10G200100， Cla97C10G201690， Cla97C10G202570， and Cla97C10G201940 were highly expressed in the resistant parent. The findings of this study provide important theoretical basis and genetic resources for marker assisted selection and breeding of gummy stem blight resistant watermelon varieties.