Rhodiola crenulata is a medicinal plant with a long history， widely used in traditional Chinese medicine and Xizang medicine. However， due to its extremely special growth environment， collection and research are very difficult， and there are currently few reports on molecular biology. This study firstly cloned the RcNAC83 gene from the Xizang Rhodiola crenulata. Through bioinformatics and real-time quantitative PCR techniques， the physicochemical properties and expression patterns of this gene were preliminarily understood. Using chromosome walking technology， the promoter sequence was predicted to identify cis-acting elements. Yeast expression vectors were constructed to verify its transcriptional activation activity， and it was ectopically expressed in Salvia miltiorrhiza to analyze the changes in phenotypes and physiological indicators under salt stress. The results showed that the coding region of RcNAC83 gene was 918 bp， containing 3 exons， and encoded 248 amino acids. The protein had obvious hydrophilicity and no transmembrane domain. It was subcellularly located in the nucleus and contained multiple phosphorylation sites. The N-terminal of the RcNAC83 gene could be divided into 5 subdomains， among which D domain was the NARD region， which had a similar amino acid sequence and conserved domain to the homologous genes， and was closely related to the genus Kalanchoe genes. The RcNAC83 promoter sequence was 888 bp and contained multiple cis-acting elements for transcriptional activation， light response， anaerobic induction， plant hormone MeJA， and recognition by MYB， MYC. RcNAC83 was expressed in roots， mature stems， stems， leaves， apical buds and flowers， and was induced by various abiotic stresses and plant hormones. This gene had no toxicity to yeast cells and transcriptional activation activity. In overexpressing Salvia miltiorrhiza， the RcNAC83 gene did not affect the growth and metabolism of Salvia miltiorrhiza， but reduced the tolerance to salt stress. This study will further enrich the biological functions of members of the plant NAC gene family， and provide a theoretical basis for revealing the molecular mechanism of highland adaptation in Rhodiola crenulata.