1.滇西科技师范学院/云南省澜沧江流域茶树资源保护与创新利用重点实验室;2.云南省农业科学院茶叶研究所;3.耿马县地方产业发展服务中心
云南省科技厅重点实验室建设项目(202449CE340010);临沧市建设国家可持续发展议程创新示范区科技专项(202204AC100001-A01)
Key Laboratory Construction Project of Yunnan Provincial Department of Science and Technology(202449CE340010);Science and Technology Special Project for the Construction of National Sustainable Development Agenda Innovation Demonstration Zone in Lincang City (202204AC100001-A01)
为鉴定和评价临沧200份古茶树种质资源表型性状遗传多样性,采用相关性分析、主成分分析及聚类分析等方法对其表型性状的遗传多样性进行研究。结果表明,28个质量性状在供试种质中表现出丰富的变异程度,各性状的遗传多样性指数存在明显差异,遗传多样性指数在0.056~1.106之间,平均遗传多样性指数为0.670;14个数量性状变异系数在5.03%~34.30%之间,平均为20.97%;相关性分析显示,大部分表型性状存在显著或极显著的相关性;主成分分析共提取出14个主成分,其累计贡献率为70.97%,分别侧重反映茶树的主脉茸毛、叶背茸毛、叶柄茸毛、花瓣颜色、叶面积、叶长、叶宽、叶片大小和叶柄长等特征;在欧氏距离20时,临沧200份古茶树种质资源可被划分为4大类群,聚类分析并未完全依地理分布划分,而主要依据表型性状的遗传多样性特征。研究结果表明,临沧市8个县(区)的古茶树种质资源表型变异丰富,遗传多样性水平较高,展现出良好的茶树良种选育潜力。本研究为临沧古茶树资源的保护与可持续利用提供了依据。
To identify and evaluate the phenotypic trait genetic diversity of 200 ancient tea tree germplasms in Lincang, correlation analysis, principal component analysis and cluster analysis were used to study the genetic diversity of their phenotypic traits. The results showed that 28 qualitative traits exhibited rich variation degrees in the tested germplasms, and the genetic diversity indices of each trait had significant differences, ranging from 0.056 to 1.106, with an average genetic diversity index of 0.670. The coefficient of variation of 14 quantitative traits ranged from 5.03% to 34.30%, with an average of 20.97%. Correlation analysis indicated that most phenotypic traits had significant or extremely significant correlations. Principal component analysis extracted 14 principal components, with a cumulative contribution rate of 70.97%, which mainly reflected the characteristics of the main vein hair, back hair, petiole hair, petal color, leaf area, leaf length, leaf width, leaf size and petiole length of tea trees. When the Euclidean distance was 20, the 200 ancient tea tree germplasms in Lincang could be divided into 4 major groups. Cluster analysis did not completely follow the geographical distribution, but mainly relied on the genetic diversity characteristics of phenotypic traits. The research results indicated that the phenotypic variation of ancient tea tree germplasms in 8 counties (districts) of Lincang was rich, and the genetic diversity level was high, showing good potential for tea tree breeding.This research provides a basis for the protection and sustainable utilization of the ancient tea tree resources in Lincang.
