As the most widely cultivated bast fiber crop globally, jute (Corchorus spp.) exhibits remarkable salt tolerance characteristics. The utilization and improvement of China"s extensive saline-alkali land resources make it highly significant to screen jute germplasm with enhanced salt-alkali tolerance for the use of these marginal lands. This study aims to systematically evaluate the salt tolerance of 100 jute germplasm accessions and identify superior materials suitable for cultivation in saline-alkali soils.The research combined indoor hydroponic experiments with field trials. Hydroponic screening was conducted at the seedling stage under NaCl concentrations of 0 (control), 200, 230, and 250 mM. Salt damage indices were assessed, and a Linear Mixed Model (LMM) was applied to analyze genotypic random effects. Breeding values for salt tolerance were estimated using Best Linear Unbiased Prediction (BLUP), based on which accessions were scored and classified. Concurrently, field trials were carried out in saline-alkali soil with a salt content of approximately 4‰. Plant height and stem diameter were measured at the technological maturity stage.The results showed that the heritability of salt tolerance in jute was 0.299, indicating a moderate level of genetic control. Sixteen highly salt-tolerant accessions were identified. Field phenotypic analysis revealed abundant genetic variation in growth traits under salt stress. Correlation analysis demonstrated a significant positive relationship between stem diameter and salt tolerance (r = 0.267, p = 0.008). Cluster analysis integrating salt tolerance, stem diameter, and plant height enabled the selection of seven elite germplasm accessions with both high salt tolerance and yield potential.This study establishes a comprehensive evaluation system for salt tolerance in jute, integrating laboratory-based seedling screening and field-based trait analysis at maturity. It provides valuable germplasm resources and a theoretical foundation for breeding salt-tolerant jute varieties and utilizing saline-alkali lands.