Dynamin-related proteins (DRPs) are a class of large molecular GTPase proteins involved in cellular activities such as cytoplasmic division, organelle fission, membrane system transport, and post-Golgi trafficking mediated by clathrin proteins. This study identified a total of 14 DRP genes in the rice (Oryza sativa L.) genome, and analyzed their physicochemical properties, conserved domains, cis-acting elements, evolutionary relationships, and expression patterns under abiotic stress using bioinformatics methods. The results indicate that OsDRP proteins are large unstable proteins, with subcellular localization in mitochondria, cytoplasm, and chloroplasts. The same subfamily are similar in the number and distribution of conserved motifs, domains, and exons, all containing Motif 1、Motif 2 and Motif 7. The OsDRP family has three large segment duplication events in its evolution, with Ka/Ks values both being less than 1, indicating purifying selection. The promoters of OsDRP genes contain cis-acting elements related to abscisic acid, auxin, low temperature, drought, light response, and plant growth and development. The qRT-PCR test indicated that the expression levels of OsDRP1E and OsDRP3A genes significantly increased under 4℃ and 20%PEG treatment, and they might be important genes in response to abiotic stress. OsDRP interacts with the clathrin adaptor complex and tetratricopeptide repeat proteins, and the binding energy of OsDRP2A and OsDRP2B is -17.4 kcal/mol. These findings provide a theoretical basis for further investigating the gene functions of the DRP family in rice growth and development.