1铜仁学院农林工程与规划学院/贵州省高等学校油茶种质资源创新与利用工程研究中心/贵州省梵净山地区生物多样性保护与利用重点实验室,铜仁 554300;2贵州省望谟县林业局,黔西南布依族苗族自治州 552399;3贵州大学贵州省森林资源与环境研究中心,贵阳 550025
研究方向为经济林栽培与育种,E-mail: trxyngyzzj@163.com
铜仁学院博士科研启动基金项目(trxyDH2324);贵州省重点实验室项目([2020]2003);2025年贵州省林木种苗培育补助项目(2025-ZM-24)
1College of Agriculture and Forestry Engineering and Planning, Tongren University/Guizhou Provincial Education Department Engineered Research Center for Innovation and Utilization of Oil Camelia Germplasm Resources/Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren 554300;2Guizhou Provincical Forestry Bureau of Wangmo County, Qianxinan Buyi and Miao Autonomous Prefecture 552399;3Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang 550025
Doctoral Scientific Research Start-up Foundation from Tongren University (trxyDH2324); Key Laboratory Project of Guizhou Province ([2020]2003); 2025 Forest Tree Seeds and Seedlings Cultivation Subsidy Project of Guizhou Province (2025-ZM-24)
以44份望谟板栗优株为研究对象,采用遗传变异分析、相关性分析、主成分分析和聚类分析对其果实表型性状进行分析,并比较不同类群数值性状的分布特征。结果表明,望谟板栗刺苞以球形、瓣裂为主,刺束颜色多为焦刺,坚果颜色以红褐色占比最高(40.91%),坚果形状以椭圆形为主(59.09%);在17个描述性状中,变异系数介于18.63%~82.31%之间,多样性指数范围为0.40~0.97。在18个数值性状中,变异系数范围为6.69%~39.58%,其中坚果宽、坚果长、果形指数、出籽率、出仁率和坚果含水量的变异系数均小于15%;遗传多样性指数介于1.43~2.19之间。主成分分析表明,前5个主成分的累计方差贡献率达80.236%。相关性分析表明,大多数数值性状之间存在显著或极显著的相关性。聚类分析将44份望谟板栗资源分为3大类,类群Ⅰ为小果类群,类群Ⅱ为中果类群,类群Ⅲ为大果类群;对聚类分析中的三类望谟板栗进行数值性状的比较分析,其中涩皮厚度、出籽率、出仁率和坚果含水量4个性状无显著差异,其余14个性状表现出显著差异。类群Ⅰ和类群Ⅱ的刺宽均存在一定离群个体比例,分别为11.54%、11.11%,而类群Ⅰ的出籽率和坚果含水率及类群Ⅱ的出仁率呈现较广的分布范围,类群Ⅲ大部分果实与刺苞大小的指标均呈现显著的向上分布趋势。本研究阐明了望谟板栗种质资源的表型多样性规律与类群分化特征,为望谟板栗品种选育奠定了基础。
This study focused on 44 elite individuals of Castanea mollissima Blume from Wangmo county, and applied genetic variation analysis, correlation analysis, principal component analysis (PCA), and cluster analysis to characterize fruit phenotypic traits, and compared the distribution patterns of quantitative traits among different groups. Results showed that Wangmo chestnut involucres (spiny bracts) were predominantly ball-shaped with valvate dehiscence, most spine bundle colors were dark brown, reddish-brown was the most common nut color (40.91%), and ellipse was the dominant nut shape (59.09%). Among the 17 descriptive traits, the coefficient of variation (CV) ranged from 18.63% to 82.31%, with the diversity index spanning 0.40 to 0.97. For the 18 quantitative traits, the CV range was 6.69% to 39.58%, among which the CVs of nut width, nut height, nut shape index, seed extraction rate, kernel recovery rate, and moisture content of nuts were all less than 15%, the genetic diversity index ranged from 1.43 to 2.19. PCA showed that the cumulative variance contribution of the first five principal components reached 80.236%. Correlation analysis indicated that significant or highly significant correlations existed among most of the quantitative traits. Cluster analysis categorized the 44 Wangmo chestnut accessions into three distinct groups: Cluster I (small-fruit group), Cluster II (medium-fruit group), and Cluster III (large-fruit group). Comparative analysis of quantitative traits among these three clusters revealed no significant differences in four traits—inner skin thickness, seed extraction rate, kernel recovery rate, and moisture content of nuts, while the remaining 14 traits exhibited significant differences. Both Cluster I and Cluster II showed a certain proportion of outliers in spine width, at 11.54% and 11.11%, respectively. Furthermore, seed extraction rate and moisture content of nuts in Cluster I, along with kernel recovery rate in Cluster II, displayed broad distribution ranges. Most indicators related to the size of the total bur and nut in Group III exhibited a significant upward distribution trend. This study elucidates the patterns of phenotypic diversity and characteristics of group differentiation in Wangmo chestnut germplasm, laying a solid foundation for the variety breeding of Wangmo chestnut.
朱周俊,吴静逸,班启明,等.望谟板栗种质资源果实表型性状多样性研究[J].植物遗传资源学报,2026,27(4):819-833.
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