Hamidazao jujube （Ziziphus jujuba ‘Hamidazao’） is a superior local cultivar exclusively in Xinjiang， China. Investigating its genetic diversity is sigficant for conserving high-quality germplasm and breeding improved cultivars. In this study， 74 elite individuals of Hamidazao jujube were evaluated through measuring 30 agronomic traits. Comprehensive assessments were conducted via variation analysis and cluster analysis， along with PCR amplification using 11 highly polymorphic ISSR primers. The results indicated that the coefficients of variation for agronomic traits among the elite individuals ranged from 5.99% to 30.09%， while genetic diversity indices varied from 1.36 to 1.95. Cluster analysis based on phenotypic traits classified the 74 accessions into five major groups at a Euclidean distance of 27. Group I exhibited strong vegetative growth vigor； Group II had significantly longer spines than the other groups； Group III showed obvious growth weakness； Group IV exhibited balanced and relatively superior growth performance； Group V comprised germplasm with high yield potential. Through the 11 ISSR primers， 94 bands were detected across the 74 elite individuals， of which 75 were polymorphic. The mean percentage of polymorphic bands per primer was 80.30%， with an average observed allele number of 1.80， an average effective allele number of 1.35， an average Nei′s gene diversity index of 0.21， and an average Shannon′s information index of 0.33. Based on the genetic similarity coefficient， the individuals were divided into five groups： Group I contained only HM56； Group II contained only HM57； Group III included HM21， HM27， HM49， and HM64； Group IV contained HM22 and HM32； and the remaining 66 accessions fell into Group V. Genetic distances among the elite individuals ranged from 0.04 to 0.52， with corresponding genetic similarities from 0.60 to 0.96. The combinations with the highest genetic similarity and the smallest genetic distance were HM7 and HM11，HM8 and HM11， while the combination with the lowest genetic similarity and the largest genetic distance is HM3 and HM56.These results suggest that considerable genetic variation and relatively rich genetic information were found among the elite individuals of ‘Hamidazao’ jujube， providing a theoretical foundation for future germplasm utilization and breeding efforts.