1吉林师范大学/吉林省植物资源科学与绿色生产重点实验室,四平 136000;2吉林省农业科学院(中国农业科技东北创新中心)/ 吉林省农业生物技术重点实验室,长春130033;3吉林师范大学博达学院,四平 136000
研究方向为大豆基因克隆和功能验证,E-mail: qi_2005@petalmail.com;
李 波,研究方向为植物抗逆胁迫机制,E-mail: libo@jlnu.edu.cn
牛 陆,研究方向为大豆生物技术育种,E-mail: niulu_0711@163.com
吉林省科技发展计划项目(YDZJ202501ZYTS503);吉林省教育厅科学技术研究项目(JJKH20250937KJ);四平市科技发展计划项目(2024058, 2024055);国家农业生物育种重大项目(2023ZD0403702)
1Jilin Normal University/Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Siping 136000;2Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China)/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033;3Boda College of Jilin Normal University, Siping 136000
Foundation projects: Jilin Province Science and Technology Development Plan Project (YDZJ202501ZYTS503); Research Projects of Science and Technology of Jilin Provincial Education Department (JJKH20250937KJ); Science and Technology Development Plan Project of Siping City (2024058, 2024055); Biological Breeding-National Science and Technology Major Project (2023ZD0403702)
为了解东北地区大豆种质资源表型性状的遗传多样性,提高大豆种质资源综合利用率,以316份大豆种质资源为试验材料,利用变异系数、遗传多样性指数及聚类分析等方法对10个农艺性状进行分析。结果表明,10个农艺性状变异系数范围为14.991%~53.045%,遗传多样性指数范围为1.705~2.929。相关性分析表明,百粒重与单株粒数负相关程度最强,株高与节间长度正相关程度最强。采用聚类分析方法将316份大豆种质划分为3个类群,类群Ⅰ含7份种质(占2.215%),其主要特征是株高优势明显,百粒重变异潜力较高,分枝数稳定性在3个类群中最优,适用于优异株型及产量育种。类群Ⅱ含12份种质(占3.797%),其核心优势是单株粒数多且稳定,株高较矮,适合密植及抗倒伏场景,分枝数多,可作为优异株型选育的基础材料。类群Ⅲ含297份种质(占93.987%),核心优势为百粒重高且稳定,叶部性状优良,株高与单株粒数均衡,分枝数变异大,种质类型丰富,可为优异品种选育提供广泛材料。基于综合得分F值筛选出10份优异种质,可作为优良亲本用于东北地区大豆育种。
In order to understand the genetic diversity of phenotypic traits and improve the comprehensive utilization efficiency of soybean germplasm resources in northeast China, this study took 316 soybean germplasm accessions as experimental materials. Ten agronomic traits were analyzed using methods including coefficient of variation (CV), genetic diversity index (H′), and cluster analysis. The results showed that the CV of the 10 agronomic traits ranged from 14.991% to 53.045%, and the genetic diversity index ranged from 1.705 to 2.929. Correlation analysis indicated that 100-seed weight exhibited the strongest negative correlation with grain number per plant, while plant height showed the strongest positive correlation with internode length. Cluster analysis divided the 316 soybean germplasms into three major groups. Group I contained seven accessions, accounting for 2.215% of all germplasms, with core characteristics of obvious plant height advantage, high variation potential of 100-seed weight, and the best stability of branch number among the three groups, making it suitable as breeding materials for excellent plant type and yield. Group II included 12 accessions (3.797% of total), characterized by abundant and stable grain number per plant, shorter plant height (suitable for dense planting or lodging-resistant scenarios), and more branches, which could provide basic materials for excellent plant type breeding. Group III consisted of 297 accessions (93.987% of total), with core traits of high and stable 100-seed weight, excellent leaf traits, balanced performance of plant height and grain number per plant, and large variation in branch number. This indicated that Group III germplasms were rich in types and could provide broader materials for elite variety breeding. Ten excellent germplasms were screened out based on the comprehensive score F-value, which could be used as excellent parents for soybean breeding in northeast China.
齐书旋,吕强,孙嘉囡,等.东北地区
