1山西农业大学园艺学院,太谷 030801;2中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室,北京 100081
研究方向为种质资源,E-mail: ma18536494557@163.com
王海平,研究方向为种质资源,E-mail: wanghaiping@caas.cn
国家现代农业产业技术体系(CARS-24-A-01);农业农村部作物种质资源安全保存项目(2025NWB037);中国农业科学院创新工程项目(CAAS-ASTIP-2025-IVFCAAS)
1College of Horticulture,Shanxi Agricultural University,Taigu 030801;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding,Beijing 100081
The China Agriculture Research System of MOF and MARA(CARS-24-A-01);The Safe Preservation Project of Crop Germplasm Resources of Ministry of Agriculture and Rural Affairs(2025NWB037); The Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2025-IVFCAAS)
为筛选高效鉴定辣椒种质资源耐盐性的方法,挖掘优异耐盐种质,本研究通过盐浓度梯度试验确定了适宜鉴定浓度,比较分析了培养皿和发芽袋两种辣椒芽期耐盐性鉴定的方法,并以最优鉴定方法对94份辣椒种质资源进行耐盐性评价。结果表明,250 mmol/L NaCl 处理可显著区分不同品种的耐盐性差异,是辣椒芽期耐盐鉴定的适宜浓度;两种鉴定方法间相对发芽率与相对发芽势无显著差异,但培养皿鉴定方法在播种效率(较发芽袋提升61.67%)与材料成本(较发芽袋降低74.59%)上优势显著,为更优鉴定方法;在 250 mmol/L NaCl 胁迫下,94份辣椒种质的发芽率、发芽指数和发芽势均受到极显著抑制(P<0.0001)。通过隶属函数法选出了新寨苗辣(A47)、青山小海椒(A37)、红灯笼辣椒(A9)、泥塘辣椒(A29)、三富村辣椒(A65)共5份芽期强耐盐品种(平均隶属函数值>0.80)。本研究明确了辣椒芽期耐盐性的高效鉴定方法,筛选出耐盐性强的辣椒种质资源,为辣椒耐盐品种选育提供了方法和材料。
In order to develop an efficient method for screening salt-tolerant pepper germplasm resources and identify superior salt-tolerant genotypes, this study determined the optimal salt concentration through gradient experiments and compared two germination-stage salt tolerance evaluation methods: petri dish and germination bag. And then, the salt tolerance of 94 varieties of pepper was evaluated by the optimal method. The results showed that 250 mmol/L NaCl treatment significantly distinguished salt tolerance differences among varieties and was suitable for germination-stage salt tolerance screening. No significant differences were observed between the two methods in terms of relative germination rate and relative germination potential, but the petri dish method demonstrated significant advantages in sowing efficiency (61.67% higher than the germination bag) and material cost (74.59% lower than the germination bag), making it the superior evaluation method. Under 250 mmol/L NaCl stress, the germination rate, germination index, and germination potential of the 94 pepper germplasm resources were all significantly inhibited (P<0.0001), and five strong salt-tolerant genotypes at the germination stage, specifically Xin Zhai Miao La(A47), Qing Shan Xiao Hai Jiao(A37), Hong Deng Long La JIAO(A9), Ni Tang La Jiao(A29), and San Fu Cun La Jiao(A65), were selected based on membership function values exceeding 0.80. This study established an efficient method for evaluating salt tolerance at the germination stage in pepper and identified salt-tolerant germplasm resources, providing both methodology and materials for breeding salt-tolerant pepper varieties.
马宁,王瑞丰,宋江萍,等.辣椒芽期耐盐性鉴定方法建立及种质资源评价[J].植物遗传资源学报,2026,27(4):776-788.
复制
