1黑龙江省农业科学院克山分院/国家马铃薯种质资源试管苗库(克山),齐齐哈尔161600;2河北农业大学农学院,保定071000
研究方向为马铃薯种质资源保护与利用,E-mail:
崔江慧,研究方向为作物遗传育种,E-mail: cjianghui521@126.com
宋继玲,研究方向为马铃薯种质资源研究与利用,E-mail: jl_song929@126.com
黑龙江省农业科技创新跨越工程农业科技基础创新优青项目(CX22YQ31);科技部、财政部国家科技资源共享服务平台项目(NCGRC-2025-44);农业种质资源保护项目(22250292,22250651)
1Keshan Branch of Heilongjiang Academy of Agricultural Sciences/Test-Tube Plantlet Bank of National Potato Germplasm Resources(Keshan),Qiqihar 161600;2College of Agronomy, Hebei Agricultural University, Baoding 071000
Foundation projects: The Excellent Youth Project for Agricultural Science and Technology Basic Innovation under the Agricultural Science and Technology Innovation Leapfrogging Program of Heilongjiang Province (CX22YQ31);Ministry of Science and Technology,Ministry of Finance National Science and Technology Resource Sharing Service Platform Project (NCGRC-2025-44); Agricultural Germplasm Resources Conservation Project (22250292,22250651)
为了分析马铃薯种质资源表型性状遗传多样性,以收集的国内外182份马铃薯种质资源为研究对象,综合运用遗传多样性指数、相关性分析、主成分分析、系统聚类等分析方法,对其表型性状进行鉴定与评价。结果表明马铃薯种质资源表型性状具有丰富的遗传多样性,描述型性状的遗传多样性指数为0.650~1.440,薯肉色的遗传多样性指数最高;数值型性状的变异系数为12.46%~31.23%,其中还原糖含量的变异系数最高,遗传多样性指数为1.885~2.091,块茎产量的遗传多样性指数最高;相关性分析表明块茎产量与分枝多少、小叶着生密集度呈显著或极显著正相关;主成分分析提取了8个主成分,累积贡献率为62.512%;聚类分析将种质资源划分为3个类群,第Ⅰ类资源平均产量最高,第Ⅱ类资源平均还原糖含量最高,第Ⅲ类资源的平均淀粉含量最高;利用隶属函数法计算综合得分,对种质资源进行综合评价,筛选出Ранний、Notach、674-5、波S、宁蒗5号、波PI、闽薯3号、78-16、78-13、NS78-6共10份优异种质资源,为马铃薯种质资源合理利用及高效研究提供了科学基础。
To analyze the genetic diversity of phenotypic traits in potato germplasm resources, a total of 182 potato germplasm accessions collected from domestic and international sources were used as research materials. Shannon-Wiener index, correlation analysis, principal component analysis, hierarchical cluster analysis and other analytical methods were comprehensively applied to identify and evaluate their phenotypic traits. The results revealed abundant genetic diversity in the phenotypic traits of potato germplasm resources. For descriptive traits, the shannon-wiener index ranged from 0.650 to 1.440, and tuber flesh color showed the highest shannon-wiener index. For numerical traits, the coefficient of variation varied from 12.46% to 31.23%, where reducing sugar content had the maximum coefficient of variation; the shannon-wiener index was 1.885 to 2.091, and tuber yield showed the highest shannon-wiener index. Correlation analysis showed that tuber yield was significantly or extremely significantly positively correlated with branch number and lobular growth density. Principal component analysis extracted eight principal components, which accounted for 62.512% of the total cumulative variance. Hierarchical cluster analysis classified all the germplasm resources into three distinct groups: group Ⅰ had the highest mean tuber yield, group Ⅱ showed the highest mean reducing sugar content, and group Ⅲ presented the highest mean starch content. The membership function method was used to calculate the comprehensive score for a comprehensive evaluation of germplasm resources, and 10 elite germplasm resources including Ранний, Notach, 674-5, Bo S, Ninglang No. 5 , Bo PI, Minshu No.3, 78-16, 78-13 and NS78-6 were screened out. This provides a scientific basis for the rational utilization and efficient research of potato germplasm resources.
张金鹏,高志昌,杨梦平,等.基于表型性状的马铃薯种质资源遗传多样性分析[J].植物遗传资源学报,2026,27(3):607-618.
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