This study was conducted using an F₈-generation recombinant inbred line （RIL） population consisting of 554 lines， developed from a cross between the edible cultivar Jihuatian 1 （maternal parent） and the low-sucrose breeding line W191 （paternal parent）. Multi-environment genetic dissection was performed to address the challenge of simultaneous improvement in both sucrose-related flavor and health-beneficial fatty acid composition in peanut. The results demonstrated high heritability for all traits investigated， with broad-sense heritability ranging from 0.76 to 0.94. Correlation analysis revealed highly significant negative correlations between sucrose content and saturated fatty acids content—stearic acid， arachidic acid， and behenic acid content（|r|=0.84-0.93）， whereas weak positive correlations were observed between sucrose content and unsaturated fatty acids content， namely oleic acid and linoleic acid content（r = 0.11-0.49）.By combining bulked segregant analysis sequencing （BSA-seq） with linkage mapping， twelve significant quantitative trait loci （QTLs） were identified on chromosomes 6 and 8. Among them， a major sucrose QTL， qSUCA06 （LOD 85.90-148.78， PVE 51.66%-66.99%）， was physically localized to the 115.68-116.06 Mb interval on chromosome 6. Notably， this interval exhibited substantial overlap with major QTLs governing three saturated fatty acids content—qSACA06 （stearic acid content）， qAACA06 （arachidic acid content）， and qBACA06 （behenic acid content）—providing direct genetic evidence for the strong negative phenotypic correlation between sucrose and these saturated fatty acids. Additionally， chromosome 8 harbored a fatty-acid QTL hotspot， in which the QTLs for oleic acid （qOACA08， LOD 6.86-32.98） and linoleic acid （qLACA08， LOD 7.49-22.07） were tightly linked but displayed opposite additive effects， consistent with the metabolic conversion catalyzed by the Δ12-desaturase （FAD2） enzyme.In summary， this research identifies key genomic regions on chromosomes 6 and 8 that coordinately regulate sucrose and lipid metabolism in peanut kernels. The findings establish a theoretical foundation and offer concrete molecular targets for marker-assisted breeding， paving the way for the development of superior edible peanut varieties that combine high sucrose content， high oleic acid， and low saturated fatty acids.