1.三明市农业科学研究院;2.海峡两岸(三明)现代林业合作实验区工作站
三明市科技计划项目(2025-N-007);2023年福建省级财政花卉产业发展项目(优良品种创新激励)(闽财资环指(2022)47号)
Sanming Science and Technology Plan Project (2025-N-007);2023 Fujian Provincial Financial Flower Industry Development Project (Excellent Variety Innovation Incentive) (Min Cai Zi Huan Zhi (2022) No. 47)
种质资源的评价及遗传多样性是现代育种的重要环节,综合性状优良且遗传背景清晰的核心种质对于建兰育种至关重要。研究以收集的109份建兰种质为材料,测定8个数量性状和9个质量性状,采用层次分析法(AHP)构建评价模型并计算综合得分;同时,利用24对SSR引物进行PCR扩增,分析群体遗传参数并进行UPGMA聚类。结果表明,花部性状是建兰观赏价值的核心(权重0.5730),其中瓣型最为关键(权重0.3350)。基于综合得分筛选出3份Ⅰ级种质(均为荷瓣)和18份Ⅱ级种质(以荷、梅瓣为主)。SSR分析检测到94个等位基因,显示群体遗传多样性丰富(PIC=0.655),且观测杂合度(Ho=0.612)高于期望值(He=0.465),表明群体可能存在一定程度的远交现象。聚类分析将群体分为7类,发现表型(AHP)与基因型(SSR)聚类部分关联但存在差异,揭示瓣型等关键观赏性状可能受少数主效基因控制,而SSR标记则反映了中性遗传背景。本研究建立的AHP评价体系明确了瓣型在建兰育种中的核心地位;SSR分析揭示群体丰富的遗传多样性及杂交活跃的背景。系统对比表型与基因型聚类结果,提出“表型初选与遗传背景优化相结合”的育种策略,为建兰核心种质筛选、分子标记辅助育种及亲本选配提供参考。
The evaluation of germplasm resources and genetic diversity are important aspects of modern breeding, and core germplasm with excellent comprehensive traits and clear genetic background is crucial for Cymbidium ensifolium breeding.Using 109 C. ensifolium accessions as materials, eight quantitative traits and nine qualitative traits were measured. An evaluation model was constructed using the Analytic Hierarchy Process (AHP) to calculate comprehensive scores. Meanwhile, PCR amplification was performed with 24 pairs of SSR primers to analyze population genetic parameters and conduct UPGMA clustering. The results showed that floral traits were the core of ornamental value (weight 0.5730), with petal type being the most critical indicator (weight 0.3350). Based on comprehensive scores, three Grade I accessions (all of lotus-type) and 18 Grade II accessions (mainly lotus-type and plum-type) were selected. SSR analysis detected 94 alleles, indicating rich genetic diversity within the population (PIC=0.655). The observed heterozygosity (Ho=0.612) was higher than the expected heterozygosity (He = 0.465), suggesting possible outcrossing events. Clustering analysis divided the population into seven groups, revealing partial correspondence but notable discrepancies between phenotypic (AHP) and genotypic (SSR) clustering. This indicates that key ornamental traits such as petal type may be controlled by a few major genes, while SSR markers reflect the neutral genetic background. The AHP evaluation system established in this study clarified the central role of petal type in C. ensifolium breeding. SSR analysis revealed rich genetic diversity and an active hybridization background within the population. Through systematic comparison of phenotypic and genotypic clustering results, a breeding strategy of "primary selection based on phenotype combined with optimization of genetic background" was proposed, providing a reference for core germplasm screening, marker-assisted breeding, and parental selection in C. ensifolium.
