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首页 > 过刊浏览>2026年第27卷第5期 >932-947. DOI:10.13430/j.cnki.jpgr.20260111001 优先出版
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茄子种质资源遗传多样性及抗病性鉴定
DOI:
10.13430/j.cnki.jpgr.20260111001
CSTR:
作者:
作者单位:

华南农业大学园艺学院/农业农村部华南地区园艺作物生物学与种质创制重点实验室,广州510642

作者简介:

研究方向为蔬菜栽培和育种,E-mail: jiazhu_feng@163.com;

通讯作者:

曹必好,研究方向为蔬菜遗传育种及蔬菜生物技术,E-mail: caobh01@scau.edu.cn

中图分类号:

基金项目:

国家自然科学基金(32472734)


Genetic Diversity and Disease Resistance Assessment of Eggplant Germplasm Resources
Author:
Affiliation:

College of Horticulture, South China Agricultural University/Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510642

Fund Project:

Foundation project: National Natural Science Foundation of China (32472734)

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    摘要:

    为系统评价茄子种质资源的遗传多样性并筛选抗病种质,以62份茄子种质为试验材料,通过形态学性状和InDel分子标记技术分析了其遗传多样性,并利用人工接种鉴定了种质对青枯病和褐纹病的抗性。结果表明,供试茄子种质的果实纵径、果形指数、果色等形态性状变异丰富,所有性状变异系数均大于10%,其中果实性状的多样性最突出。表型聚类将供试种质划分为4个类群,依据果实性状可区分紫红长茄、白绿果茄等类型;InDel分子标记聚类同样划分为4个类群,其中栽培种高度集中于第Ⅰ类群(49份),遗传基础相对狭窄,而野生种独立聚为第Ⅲ、Ⅳ类群,与栽培种遗传距离较远,是拓宽遗传多样性的关键来源。在分子水平上,23个InDel标记的多态性信息含量(PIC)均值为0.290,其中18个标记(78.3%)表现为中度多态性(0.25<PIC<0.50),5个标记为低度多态性(PIC<0.25),表明遗传多样性处于中等水平。抗病性鉴定共筛选出4份高抗青枯病材料,包括野生种质非洲红茄(24AE001、24AE002)和水茄(24AE004)以及栽培种质24AE100;8份免疫褐纹病材料,包括野生种质非洲红茄(24AE001、24AE002)、喀西茄(24AE003)以及栽培种质24AE024、24AE058、24AE068、24AE078、24AE098,其中野生种质表现出更强的抗病性。本研究结果表明茄子种质资源具有较丰富的遗传多样性,野生种质资源是重要的抗病基因来源,筛选出的抗病材料可为茄子抗病育种提供重要的种质基础。

    Abstract:

    To systematically evaluate the genetic diversity of eggplant germplasm resources and screen for disease-resistant germplasms, this study used 62 eggplant accessions as experimental materials. Genetic diversity was analyzed through morphological traits and InDel molecular marker technology, and resistance to bacterial wilt and phonopsis blight was identified via artificial inoculation. The results showed that the morphological traits of the test eggplant accessions,such as fruit longitudinal diameter, fruit shape index, and fruit color,exhibited rich variation, with coefficients of variation for all traits exceeding 10%, among which fruit traits showed the most pronounced diversity. Phenotypic clustering divided the tested germplasms into four groups, primarily distinguishing types such as purple-red long eggplant and white-green fruit eggplant based on fruit traits. InDel molecular marker clustering also resulted in four groups, with cultivated varieties highly concentrated in Group Ⅰ (49 accessions), indicating a relatively narrow genetic base, while wild species were independently clustered into Group Ⅲ and Ⅳ, showing a large genetic distance from cultivated varieties and serving as a key source for broadening genetic diversity. At the molecular level, the mean polymorphism information content (PIC) of the 23 InDel markers was 0.290, with 18 markers (78.3%) exhibiting moderate polymorphism (0.25<PIC<0.50) and five markers showing low polymorphism (PIC<0.25), indicating a moderate level of genetic diversity. Disease resistance evaluation identified four accessions highly resistant to bacterial wilt, including the wild germplasm African scarlet eggplant (24AE001, 24AE002) and turkey berry (24AE004) , as well as cultivated accession 24AE100; and eight accessions immune to phomopsis blight , including the wild germplasm African scarlet eggplant (24AE001, 24AE002) and Kashi eggplant (24AE003) , as well as cultivated accessions 24AE024, 24AE058, 24AE068, 24AE078, and 24AE098. Wild germplasm accessions exhibited superior disease resistance. These findings confirm that eggplant germplasm resources possess substantial genetic diversity, with wild germplasm serving as an important reservoir of disease resistance genes. The identified resistant germplasms provide valuable genetic resources for disease-resistant breeding programs.

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冯家珠,李焯韵,邱晓雨,等.茄子种质资源遗传多样性及抗病性鉴定[J].植物遗传资源学报,2026,27(5):932-947.

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  • 收稿日期:2026-01-11
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  • 在线发布日期: 2026-05-12
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