华南农业大学园艺学院
国家自然科学基金项目(面上项目,重点项目,重大项目)
College of Horticulture, South China Agricultural University
The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)
为系统评价茄子种质资源的遗传多样性并筛选抗病材料,本研究以62份茄子种质为试验材料,通过形态学性状和InDel分子标记技术分析了其遗传多样性,并利用人工接种鉴定了其对青枯病和褐纹病的抗性。结果表明,62份茄子种质材料形态性状变异丰富,所有性状变异系数均大于10%,其中果实性状的多样性最突出。在分子水平上,23个InDel标记的多态性信息含量(PIC)均值为0.290,表明遗传多样性处于中等水平。抗病性鉴定共筛选出4份高抗青枯病材料,包括野生种质非洲红茄(24AE001、24AE002)和水茄(24AE004) 以及栽培种质24AE100;8份抗褐纹病材料,包括野生种质非洲红茄(24AE001、24AE002)、喀西茄(24AE003) 以及栽培种质24AE024、24AE058、24AE068、24AE078、24AE098,其中野生种质表现出更强的抗病性。本研究结果表明茄子种质资源具有较丰富的遗传多样性,野生种质资源是重要的抗病基因来源,筛选出的抗病材料可为茄子抗病育种提供重要的种质基础。
To systematically evaluate the genetic diversity of eggplant germplasm resources and screen disease-resistant germplasms, this study analyzed 62 eggplant accessions using morphological traits and InDel molecular markers, combined with artificial inoculation to assess resistance to bacterial wilt and brown rot. The results showed that the morphological traits of the tested materials exhibited high variation, with all trait variation coefficients exceeding 10%, among which fruit traits showed the most pronounced diversity. At the molecular level, the mean polymorphism information content (PIC) of 23 InDel markers was 0.290, indicating a moderate level of genetic diversity. Disease resistance evaluation identified 4 accessions highly resistant to bacterial wilt, including the wild germplasm African scarlet eggplant (24AE001, 24AE002) and turkey berry (24AE004) , as well as cultivated accession 24AE100; and 8 accessions immune to brown rot, including the wild germplasm African scarlet eggplant (24AE001, 24AE002) and Kashi eggplant (24AE003) , as well as cultivated accessions 24AE024, 24AE058, 24AE068, 24AE078, and 24AE098. Wild germplasm accessions exhibited superior disease resistance. These findings confirm that eggplant germplasm resources possess substantial genetic diversity, with wild germplasm serving as an important reservoir of disease resistance genes. The identified resistant germplasms provide valuable genetic resources for disease-resistant breeding programs.
