NAC transcription factor is one of the largest gene families in plants, playing a critical role in plant growth, development and stress response. The NAC transcription factor family in cabbage was systematically identified and the expression features of its members in various tissues and organs, and under high temperature stress were analyzed, thus providing a theoretical basis for further exploring the function of this gene family under stress condition in cabbage. NAC proteins in cabbage were screened using HMMER software and validated by the InterPro and NCBI-CD Search online websites. The physicochemical properties, chromosome distribution, gene structure, and phylogenetic relationships of NAC gene family members in cabbage were analyzed by using tools such as ProtParam, TBtools, MEME and MEGA. Meanwhile, the expression patterns of cabbage NAC genes under different tissues and high temperature stress were examined based on transcriptomic data and quantitative real-time PCR. The subcellular localization of the BoNAC36 protein was analyzed using a transient expression system in Nicotiana benthamiana leaves. The 204 BoNAC genes were identified from the cabbage genome. The amino acid lengths ranged from 154 to 855 amino acids, with molecular weights ranging from 17.75 to 95.18kD, and isoelectric points between 4.33 and 9.83. The 204 BoNAC genes were unevenly distributed across nine chromosomes, with the highest number of genes located on chromosome 5. Bsaed on the the Arabidopsis NAC gene grouping method, BoNAC genes were divided into 16 subgroups, with members within the same subgroup exhibiting similarities in both conserved motifs and gene structures. The promoter regions of BoNAC genes contained multiple cis-acting elements related to light response, hormone response, stress response, and growth regulation. Tissue-specific expression analysis showed that genes highly expressed in roots belonged to the TIP and ATAF subgroups. The BoNAC36, BoNAC41, BoNAC69 and BoNAC111 genes were significantly expressed under high temperature stress treatment. Subcellular localization results indicated that BoNAC36 was localized in the nucleus. This study provides important data support for the in-depth analysis of the function of BoNAC genes in cabbage.