Ratooning ability is an important agronomic trait for rice varieties. Rolling tolerance reflects the resilience size of rice stubble after the main crop harvested by machine. Accurately selecting rice varieties with high rolling tolerance and strong ratooning ability is crucial for the mechanisation of the whole rice production process. However, in ratoon rice production, the rolling tolerance and ratooning ability are differed largerly among different rice varieties, and also, there is a lack of annual high-yielding ratoon rice varieties suitable for mechanical harvesting. Consequently, these factors led to low and unstable ratoon rice yields under the mechanisation of the whole rice production process. Addressing this issue, based on several years of field experiments, this paper observed the pushing resistance, ratooning ability , and comprehensive resistance among different rice varieties, and further comprehensively summarized the technical methods and operational processes for evaluating the rolling tolerance and strong ratooning ability of ratoon rice varieties. . This paper formulated technical specifications covering field planting, measurement methods, evaluation methods, and determination rules for mechanically harvested ratoon rice, clarifying the technical standards for suitable ratoon rice selection. The aim is to provide standardized technical support for the precise selection of annual high-yield ratoon rice varieties suitable for mechanical harvesting and the identification of related germplasm resources. This protocol is applicable to the selection and evaluation of mechanically harvested ratoon rice varieties in Chongqing and similar ecological regions upstream of the Yangtze River.