Relationships and parental specific markers among sugarcane germplasm were analyzed with RAPD. 25 arbitrary primers with polymorphism were selected and used to make the RAPD fingerprinting of 41 sugarcane germplasm. The RAPD data were clustered using UPGMA method. It showed that genetic diversity existed among sugarcane cultivars and relative species. But the genetic similarities were high among these sugarcane germplasm, indicating their close relationships. Some specific RAPD markers were amplified from some sugarcane parental species with some certain arbitrary primers. The parental specific RAPD markers could be observed at a higher frequency in their progenies.