The powdery mildew resistance genes in 11 tetraploid wheat-Aegilops amphidiploids, Triticum carthlicum acc. PS5, Triticum durum acc. DR147, 4 Aegilops accessions and advanced lines Am9/Laizhou953*2 F 5 and (DR147/Ae14)//Laizhou953*2 F 4 were analyzed by inoculating detached primary leaf segments with a set of 20 differential powdery mildew isolates. By comparisons of the response pattern of differential wheat cultivars or lines and application of the gene-for-gene hypothesis, we deduced that resistance gene Pm4b occurred in line Am9/Laizhou953*2 F 5, and an unknown resistance gene in combination with resistance gene Pm4b occurred in T. carthlicum acc. PS5. Line (DR147/Ae14)//Laizhou953*2 F 4 and T. durum acc. DR147 possessed an unknown resistance gene in combination with resistance gene Pm4a,respectively Ae. caudata acc. Ae14 and Ae. umbellulata acc. Y39 showed resistance to all the isolates tested, they should carry a novel resistance gene because no powdery mildew resistance gene has been introgressed into common wheat from Ae. caudata and Ae. umbellulata. Except in Am9, the resistance of T. carthlicum acc. PS5 or T. durum acc. DR147 was in part suppressed in an amphidiploid, the resistance of Aegilops accession was completely, or in part, suppressed in an amphidiploid.