Genetic diversity is important for breeding programs. Thirty-two selected microsatellite markers were used to detect genetic diversity among 80 salt-tolerant wheat cultivars. Totally 155 alleles were revealed among the 80 accessions. The number of alleles per SSR loci varied from 1 to 12, most of them from 4 to 8 and with the average of 4.75. The genetic distance among the 80 accessions varied from 0.26 to 0.81. Cluster analysis among the 80 accessions with salt tolerance showed that all the cultivars were clustered into two groups, that is the winter wheat group and the spring wheat group. Most accessions with same pedigree backgrounds were clustered into one subgroup. The difference of allelic variation in genome A, B and D was small, but genome B had the highest number of alleles.