The genetic integrity of 20 wheat accessions from different propagation times was studied by PAG-electrophoresis. The result showed that there were 10 genetically homogeneous accessions having only one gliadin spectrum biotype and 10 genetically heterogeneous accessions having 2 - 4 gliadin spectrum biotypes. Among 10 genetically heterogeneous accessions, 6 were landraces. It showed that landraces had high heredity variation. The germination rates before regeneration were higher than 75 % for the 5 genetically heterogeneous accessions in which the differences of frequency of gliadin spectrum biotypes were not significant between the two populations propagated in different years. But for other 5 genetically heterogeneous accessions, the differences of frequency of gliadin spectrum biotypes were significant between the two populations propagated in different years and their germination rates were lower than germination rate was positively correlated with difference of heterogeneous accessions, and the correlation coefficient was germination rate was the main factor for maintaining the genetic 66 %. The result also showed that the difference of the frequency of biotypes at 1% level of 10 genetically 0.8665. The result indicated that the propagation integrity of heterogeneous germplasm