Random amplified polymorphic DNA (RAPD) markers were used to estimate genetic diversity of Liriodendron. Fourteen primers generated 235 bands from 131 samples. It was indicated that Liriodendron has high genetic diversity. (1)The polymorphic bands percentage of L.chinense (Hemsl.)Sarg., L.tulipifera L. and hybrid clones ware 88.98%,91.06% and 89.98% ,respectively. (2)The genetic variation was observed within populations. (3)Clustering data indicated that the hybrid clones is close to L. tulipifera L. in heredity. Basic data were provided for further studies on the genetic variation, selection and evaluation of Liriodendron.