Four lines including Jinhan125,Xiye501 and their cross offspring were selected as test materials. Physiological and ecological indexes relevant to drought-tolerance in seedling and flower-podding stage were determined by laboratory testing and field observation. In the condition of normal water supply and drought stress,the flower-podding stage was the most sensitive stage to water content in whole stages. We determined relative water content,ability of water holding,proline content,activity of POD,SOD,EST and analyzed their bands. The result indicated that the order of drought-tolerance was as follows: Jinhan125,73-1,73-2,Xie501,73-3,73-4.