The gliadin composition of seventy-two Chinese wheat(Triticum aestivum L.) landraces was analyzed by A-PAGE in order to understand their genetic heterogeneity and genetic diversity.In total,101 gliadin bands were observed in all accessions,which formed 229 gliadin patterns.The number of gliadin bands in a landrace ranged from 14 to 24.Sixty-three landraces (87.5%) had more than two types of combinations of gliadin patterns.Among them, Erhongpi(ZM004659) had the highest number of 14 gliadin patterns in 30 kernels.Most landraces had two to three gliadin banding patterns.Nine landraces(12.5%) had identical gliadin patterns in different kernels within each entry.This indicates that most Chinese landraces examined were genetically heterogeneous in gliadin constitution.Cluster analysis demonstrated that the landraces originated from similar ecotypes were not grouped together.