Two methods,toothpick inoculation and silk channel inoculation,were compared for screening the maize germplasm resistance to Fusarium ear rot.The results indicated that toothpick inoculation caused higher severity of maize ear comparing with that of silk channel inoculation.The toothpick inoculation was a desired and easy to operate technique for screening the resistance,the pathogen in between kernel and cob was favor infection and promoted establishment of the pathogen.A total of 178 maize inbred and 15 hybrids were evaluated for resistance to ear rot by using toothpick inoculation in the field.Among the tested maize inbred and hybrids,one highly resistant and 34 resistant maize inbred,and 12 resistant hybrids were screened for the disease resistance to Fusarium ear rot.