In vitro shoot-tips of banana(Musa spp.)were successfully cryopreserved by droplet vitrification method.The factors affecting the post-thaw regeneration of shoot-tips were analyzed.The results showed that the droplet vitrification method was more suitable for cryopreservation of banana in vitro shoot-tips than vitrification method.The optimal procedures were as follows:Shoot-tips with 1 or 2 leaf primordia excised from rooted in vitro plantlets which were cultured on MS medium supplemented with 60g/L sucrose for 1～2 months.30min loading time could be prolonged up to 4h at room temperature,and the optimal treatment time of PVS2 was 40～50min at 0℃.The average post-thaw regeneration rate was 46.9% of 14 accessions belonging to six different genomic groups of Musa spp.after cryopreservation by droplet vitrification method.There was no variation in the regenerated plantlets on SSR molecular level