国家重点基础研究发展计划项目(2006CB101700);国家高技术研究发展计划项目(2006AA100201);国家自然科学基金(30730063)
以5个抗旱性不同的玉米骨干自交系黄早四、掖478、郑58、旱21、齐319为试验材料,对持续水分胁迫下玉米幼苗的叶片相对含水量、渗透调节能力(OA)、脯氨酸含量和可溶性糖含量的变化规律进行了研究。结果表明:随着干旱胁迫的加重,叶片相对含水量呈现下降的趋势,渗透调节能力和可溶性糖含量呈现先上升后又下降的趋势,脯氨酸含量呈现持续上升的趋势;在持续干旱条件下,不同自交系各个指标的变化幅度不同,说明不同种质资源对干旱胁迫的响应方式不同,渗透调节能力也有差异。在水分胁迫的前7d中,渗透调节能力逐渐增加,第7天时达到最大值,OA从大到小的排列顺序为齐319>郑58>掖478>旱21>黄早四;但此后,除旱21外,其余4个自交系的OA都随之下降。旱21和齐319以可溶性糖和脯氨酸为主要的渗透调节因子,黄早四则以脯氨酸为主要的渗透调节物质。
Changes of relative water content(RWC),osmotic adjustment capacity(OA),proline content and soluble sugar content in leaves of five important maize inbred lines(i.e.Huangzaosi,Ye 478,Zheng 58,Han 21,and Qi 319)under drought stress were investigated.The results showed that RWC decreased,OA and soluble sugar content firstly increased and then decreased,and proline content increased gradually under drought stress.Moreover,the traits changed differently among these inbreds,indicating that different germplasm responded to drought stress differently.For instance,they had different OA capacity under drought.During the first seven days,OA increased gradually,with the order of Qi 319,Zheng 58,Ye 478,Han 21 and HuangzaosiHowever,OA decreased afterwards in all of the inbred lines except Han 21Soluble sugars and proline were the major osmolytes in Han 21 and Qi 319,while proline was the major osmolyte in Huangzaosi
裴二芹,石云素,刘丕庆,等.干旱胁迫对不同玉米自交系苗期渗透调节的影响[J].植物遗传资源学报,2010,11(1):40-45.
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