Zinc-finger proteins play a key role in the regulation expression of defense genes and resistance response of plant. So far, few articles reported C3HC4-type RING zinc-finger protein genes in the soybean. In this study, a RING zinc-finger protein gene was isolated from soybean (Glycine max, Tiefeng 8) cDNA library treated by drought stress for 5 h using NTT system. This gene contains 927 bp, coding 308 amino acids, and has a C3HC4-type RING finger domain and named GmRZFP1. Phylogenetic analysis showed that GmRZFP1 belong to C3HC4-type Zinc-finger subfamily. Real-time PCR results showed that the expression of GmRZFP1 gene was induced by drought, high salt, high temperature, low temperature, ethylene and ABA stresses, suggesting that GmRZFP1 might involve in some stresses related signal pathways. Subcellular localization assay indicated that the 163GFP-GmRZFP1 fusion protein was located in the nucleus of plant. The results would be useful in the study of functions of this gene family in stress responses of soybean, and clarify molecular mechanisms of stress resistance in soybean.