The present research was focused to analysis phenotypic genetic diversity among 200 accessions of geographically different cultivars of G. arboreum in National Medium-term Gene Bank of Cotton in China. The result indicated that, the phenotypic genetic diversity was abundant in G. arboreum L. cultivars. There was significant or highly significant variation among 200 accessions based on each phenotype trait. Shannon-Wiener index（H`）of the 19 phenotypic traits ranged from 0.34 to 2.15, the orders as below: lint percentage>plant height>growth period>boll weight>sympodial branch node=seed index>monopodial branch number>seed fuzz color>Verticillium wilt resistance>stem color>stem pubesence amount>leaf color>petal base spot color>leaf shape=leaf base spot color>corolla color>seed fuzz>leaf nectary>fiber color. The phenotypic diversity of the four cotton-growing areas from higher to lower was Yangtze River region, Yellow River region, South China region and foreign countries. Software NYSTS-pc2.20 and UPGMA were used for the cluster analysis on genetic distance matrix. The result showed that, the Euclidean distance among all the 200 G. arboreum accessions ranged from 0.85 to 11.72(average 5.92). The 200 accessions were clustered into 16 phenotypic types when the value was 5.48, such as types of green broad leaf, red leaf, okra leaf, brown fiber, early maturity, late maturity, big boll, high lint percentage and so on, and 8 accessions with specific phenotypic traits were selected. No apodictic relationships were observed between genetic distance and geographic distance.