During last decades, C. sojae become more severe in the Heilongjiang soybean production region due to the change of the soybean cultivars and clmiate. To obtain some resistant to C. sojae in soybean production and breeding, we evaluate and appraise the resistance of C. sojae from 1073 portions of Soybean resource under artificial infection condition. The results showed that there were 33 portions of high resisted resource, 291 portions of resisting disease, 332 portions of moderate resistance、381 portions of affecting disease, and 36 portions of highly affecting resource, which respectively occupied 3.1%、27.1%、30.9%、35.5%、3.4%. We also found much higher frequent advantage of physiological seeds from Number 1, 6 and 7 were 161 portions, 68 portions, 95 portions, which occupied 15%、6.3%、8.9% respectively. All these results showed that there are plenty of anti-disease sources in recent soybean germplasm resources.