A new wheat derivative line 3558-2 from the cross between common wheat variety Fukuho and Agropyron cristatum Z559, possess the elite traits of more spikelet number per spike, kernel number per spikelet and kernel number per spike. In order to reveal the genetic feature of spike traits in line ‘3558-2’, a population derived from the cross between ‘3558-2’ and ‘Jing 4841’ was used to genetic analysis and QTL mapping about spike length, spikelet number per spike, kernel number per spikelet and kernel number per spike traits. The analysis results of the major gene plus polygene model indicate that these spike traits are all accord with the feature of quantitative traits. QTL mapping results show that four QTLs were detected on chromosomes 1A, including spike length, spikelet number per spike, kernel number per spikelet, kernel number per spike, which explain 14.41%, 5.15%, 14.84% and 10.87% of phenotypic variance respectively. Interestingly, an elite gene clusters were found on 1AS chromosome contained the traits of spike length, spikelet number per spike, kernel number per spikelet, kernel number per spike. The results suggest that line ‘3558-2’ carry a elite gene cluster on 1AS chromosome , and also be a good germplasm resource for wheat improvement.