Microspore developmental stage and morphology of flower organ were studied by both of anatomical characteristics and morphological methods in Chinese jujube (‘Zanhuangdazao’, ‘Junzao’, ‘Pingguozao’, ‘Lizao’) and wild jujube. The results showed that buds color of microspore late-uninucleate were yellow-green, the color of anthers were light yellow, flower bud diameter of ‘Zanhuangdazao’ was about 2.7 mm, height was about 1.6 mm; flower bud diameter of ‘Junzao’, ‘Pingguozao’ and ‘Lizao’ were about 2.0 mm, height was between 1.1 mm and 1.8 mm; flower bud diameter of ‘Zanhuangdazao’ was about 1.9 mm, height was about 1.0 mm. We can judge the development stages of microspore by morphological characteristics of flower organs so as to define the standard of bud selection in correspondence with the optimal stage of anther culture or microspore culture.