Carnation is one of the world's four cut flowers with important ornamental value. Its germplasm resources are mainly conserved in field genebanks and in vitro genebanks, which including tissue culture and cryopreservation. As complementary to conservation in field genebanks, in vitro genebanks can conserve the germplasms in short, medium and long-term. In this paper, the related researches in the carnation germplasms in vitro conservation were studied summarily, aimed at providing references to carnation germplasm conservation.