1066 watermelon accessions were screened for drought resistance under progressive drying condition at seedling stage. The result indicated that the suffered degree was significantly different among accessions. Using fast cluster analysis, 1066 collections were devided into 4 classes, which were resistant, susceptible, middle resistant and resistance seperation, respectively. 25 accessions were identified as high resistant to drought, while 30 collections were middle resistance. Most of high resistant accessions were wild germplasm which from Africa, they were suitable for watermelon rootstock breeding. Those middle resistant germplasms were cultivar accessions, which have good agriculture traits and disease resistance, they could be used as watermelon drought resistance breeding.