基于SSR标记的寒地水稻品种骨干亲本分析
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国家科技支撑计划项目(2007BAD65B01);省科技攻关计划重大项目(GA09B102-1);东北农业大学创新团队项目(CXT001-2-1)


Analysis of Main Parent in Cold Rice by SSR Marker
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    摘要:

    富士光、藤系138、上育397和五优稻1是20世纪90年代至今寒地水稻品种选育的骨干亲本。利用50对SSR引物对上述骨干亲本及其衍生品种进行聚类分析和主坐标分析(principal coordinate analysis, PCO),结果表明,50对SSR引物在51份供试材料中共检测到150个等位基因,变化范围为2~6个,平均为3个;引物PIC的变化范围为0.0725~ 0.6845,平均0.3655;聚类分析将51份材料分为4类,4个骨干亲本分别被聚到4类中;PCO分析显示,四个骨干亲本相距较远,呈独立的分支,衍生品种围绕着骨干亲本分布;在检测出的39个稀有等位基因中,仅有3个存在骨干亲本中。表明近年寒地水稻品种遗传改良是围绕少数骨干亲本进行的,骨干亲本将大部分优良基因传递到了衍生品种中,SSR分析和PCO分析与系谱分析得到了一致的结果。

    Abstract:

    Fushiguang, Tengxi138, Shangyu397 and Wuyoudao1 are the founder parents of rice breeding at cold region from the 1990s. 50 SSR markers were used to cluster and PCO analyze the the founder parents and their derivatives. The results showed that 150 alleles were detected and allelic variation of single SSR loci ranged from 2-6, mean allelic variation was 3; PIC ranged from 0.0725-0.6845, mean 0.3655; 51 experiment varieties were separated into four classes and four founder parents were in four classes separately; four founder parents were far from each other and their derivatives distributed around them through PCO analysis. Only 3 in 39 rare alleles detected existed in founder parents. This showed that genetic improvement of cold rice has progressed to surround a few founder parents in recent years. Founder parents has transmitted their most fine genes to derivatives and pedigree analysis has the same result with SSR and PCO analysis.

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刘化龙.基于SSR标记的寒地水稻品种骨干亲本分析[J].植物遗传资源学报,2011,12(6):865-871.

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  • 收稿日期:2011-04-14
  • 最后修改日期:2011-05-13
  • 录用日期:2011-08-25
  • 在线发布日期: 2011-09-23
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