Herbicide resistance of seedling and combining ability of 10 main agronomic traits have been analyzed for 30 hybrids mated by NCⅡ designation using 6 herbicide-resistant indica restorer lines as male and 5 wide-utilized sterile lines as female. The resistance results showed that restorer lines and three-line hybrids had mostly complete resistance of herbicide, and two-line hybrids had more than 90% resistance level. The combination analysis showed the difference of GCA between female parents reached the significant level for all traits but PN, all for male parents. Four traits (YP, SSR, KGW, and DS) showed the significant difference among SCA effects of combinations. Among the sterile lines, Jinke 1A had the highest GCA effect in such traits as YP, SSR, and so on, but VSCA was the lowest; Guangzhan 63-4S had the highest GCA and VSCA of KGW, most negatively effect of GCA in DS; C815S had the most negative effect of GCA and higher VSCA in PH. Among the restorer lines, Huakanghui 101 had the highest GCA and VSCA in PN; Huakanghui 104 had the highest GCA in PL, the highest negative effect in DS; Huakanghui 105 had the highest GCA in YP, SSR and so on, and negative GCA effect in PH; Huakanghui 106 had the highest GCA and VSCA in KGW. Some herbicide-resistant hybrids with strong heterosis could be obtained using herbicide-resistant restorer lines as male through comprehensive mating of parents with high GCA and VSCA.