Abstract:Diversity of cytoplasmic male sterility is key measure for solving grain quality, resistance and yield of three line hybrids. Sterile plants H236A was found in offspring from Miyang 46 as female parent. Its sterile type and abortion degree were determined by hybridization and selfing; Its pollen grain fertility, abortion shape, and abortion stage were studied by 1%I2-KI staining and observation of Leica fluorescence microscope DM 2500 on mature pollens. Results showed that H236A was cytoplasmic male sterility, which abortive degree was above 99.8%. Pollen grains belong to typical abortive was 83.17% and round abortive was 16.83%, no stained abortive pollen grains. Abortive stage of H236A was in single nuclear development period. Abortive shapes of pollen grains were in varied forms, such as irregular, round and spindle etc. Phenomenon that cytoplasm of late microspore moved directionally and formed cytoplasm bridge was observed clearly. Division of mature pollens abortive stage and shape were also discussed in this paper.