Light-intensity-affected fertility of thermo-photo-sensitive genic male sterile (TGMS) wheat (Triticum aestivum L.) lines C412S and C404S, which were reported to be sterility under lower temperature condition and normal fertility under higher temperature condition, was investigated in growth chambers, with normally fertile wheat lines C412 and C404, the recurrent parents of C412S and C404S, as control. The plants were treated during the developmental period from pollen mother cells (PMCs) formation stage to the anthesis stage in growth chambers at two temperature levels ( 8℃ day temperature, 6℃ night temperature, and 18℃ day temperature, 14℃ night temperature) and two light intensity levels ( 160 and 300 μmol /m2·s) with photoperiod 12 hours day time and 12 hours night time. The results showed that under lower temperature condition (8 ℃ day temperature and 6℃ night temperature), both C412S and C404S showed complete sterility with self-fertilized seed setting rate of 0 % whether at higher light intensity level (300 μmol /m2 ·s) or at lower light intensity level (160 μmol /m2 ·s) . Meanwhile, under higher temperature condition (18℃ day temperature and 14℃ night temperature), C412S showed highly sterility with self-fertilized seed setting rate of 5.4 % after being treated with lower light intensity of 160 μmol /m2·s, but showed normal fertility with self-fertilized seed setting rate of 65.0 % after being treated with higher light intensity of 300 μmol /m2·s; and on the other hand, C404S showed normal fertility whether under higher light intensity (300 μmol /m2 ·s) or under lower light intensity (160 μmol /m2 ·s), with self-fertilized seed setting rate of 69.9 % and 73.2 % after being treated with light intensity of 160 and 300 μmol /m2·s, respectively. These results suggested that light intensity had important effect on fertility alternation of TGMS wheat.