The distinctness of 25 commercial cultivars was identified with SSR marker from 14 pairs of primers for Lentinula edodes. The polymorphism was 100% for the all of primers. The 2-9 alleles were generated for one primer pair and the 5.0 alleles was average to each primer pair. The 2-12 genotypes were formed for one primer pair and the 6.3 genotypes was average to each primer pair. The expect heterozygosity 0.1151-0.8131 was presented and with average 0.6216. The PIC values ranged from 0.1064 to 0.7736 with average 0.5541. The 23 varieties were separated clearly each other by the 14 primer pairs except the one cluster consisted of ShenXiang 10 and ShenXiang 12. This result provided the basic technique for making SSR fingerprint profile to identify Lentinula edodes cultivars. The SSR profile is suitable to standard fingerprint for identification of the cultivars and it is good repeated and easy contrasted with various operators and labs compared with the profiles from RAPD, ISSR and SRAP. The operator no longer puts all of the presented cultivars as reference to be tested in the identification so that the workload reduced greatly.