Determination on pollen viability of 19 pear cultivars in different storage temperatures were carried out by testing method of pollens cultured in vitro, pollination characteristics of 6 cross combinations and inbred of 15 pear cultivars were also studied. The results showed that addition of boric acid and gibberellin in culture medium can enhance pollen viability. It was found that Daci pear,Bayuehong,Whangkeumbae pear pollen abortion assayed under microscope.In different storage conditions, the lower of the temperature, the more slowly of the pollen germination rate dropped, lower temperature was suitable for long storage. During process of storage, the appearance of “compelled dormancy of short-term” was exist, and with lower temperature forced dormancy recovery time backward outspread. The average of fruit setting rate of flower and inflorescence of 6 cross combinations were 82.54% and 94.82%, and had no significant differences with open pollination. Gold-Nijisseiki could be used as pollination tree for Ya pear and Xuehua pear, also Xuehua pear, Ya pear, Red D' Anjou and Red clapp's favorite could be used as pollination tree for Whangkeumbae.The self-fertile rate of 15 pear cultivars were low, and 8 cuitivars included Daci pear, Whangkeumbae, Qinfeng, Xiuyu, Sinsei, Xuehua pear and Housui had no self-fertile rate. In production, pollination tree were needed to achieve production demand.